Effect of opioids on tissue metabolism in aestivating and active Green-Striped Burrowing Frogs, Cyclorana alboguttata

Kayes, Sara M., Cramp, Rebecca L., Hudson, Nicholas J. and Franklin, Craig E. (2013) Effect of opioids on tissue metabolism in aestivating and active Green-Striped Burrowing Frogs, Cyclorana alboguttata. Journal of Herpetology, 47 2: 369-377. doi:10.1670/12-039


Author Kayes, Sara M.
Cramp, Rebecca L.
Hudson, Nicholas J.
Franklin, Craig E.
Title Effect of opioids on tissue metabolism in aestivating and active Green-Striped Burrowing Frogs, Cyclorana alboguttata
Formatted title
Effect of opioids on tissue metabolism in aestivating and active Green-Striped Burrowing Frogs, Cyclorana alboguttata
Journal name Journal of Herpetology   Check publisher's open access policy
ISSN 0022-1511
1937-2418
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1670/12-039
Open Access Status
Volume 47
Issue 2
Start page 369
End page 377
Total pages 9
Place of publication Salt Lake City, UT, United States
Publisher Society for the Study of Amphibians and Reptiles
Language eng
Abstract Metabolic depression is a common strategy used during stressful environmental conditions, allowing animals to survive for prolonged periods on limited endogenous fuel stores; yet, the mechanisms involved in regulating metabolic depression are poorly understood. The endogenous opioid system (EOS) has been implicated in the regulation of metabolism during hibernation in vertebrates, and we hypothesized that opioids also may regulate metabolic rate during aestivation in the Green-Striped Burrowing Frog, Cyclorana (Litoria) alboguttata (Gunther, 1867). When incubated in the presence of opioid agonists, liver and muscle tissue slices from C. alboguttata underwent tissue- and agonist-specific metabolic depression, and the effect of opioid agonists on metabolism was more pronounced in tissue slices from aestivating animals. Gene transcript levels for the delta-opioid receptor remained constant in the brain and muscle of aestivating C. alboguttata compared with that of active frogs, as did brain transcript levels of the opioid receptor XOR1. The results of this study demonstrate that opioids can influence metabolic rate in isolated tissues from C. alboguttata. The maintenance of key opioid receptor gene transcript levels in tissues of aestivating frogs suggests that despite the energetic costs, maintenance of receptor abundance is important. We propose that the maintenance of opioid receptor abundance during aestivation in C. alboguttata is indicative of a role for the EOS in the regulation of metabolic rate during aestivation.
Formatted abstract
Metabolic depression is a common strategy used during stressful environmental conditions, allowing animals to survive for prolonged periods on limited endogenous fuel stores; yet, the mechanisms involved in regulating metabolic depression are poorly understood. The endogenous opioid system (EOS) has been implicated in the regulation of metabolism during hibernation in vertebrates, and we hypothesized that opioids also may regulate metabolic rate during aestivation in the Green-Striped Burrowing Frog, Cyclorana (Litoria) alboguttata (Gunther, 1867). When incubated in the presence of opioid agonists, liver and muscle tissue slices from C. alboguttata underwent tissue- and agonist-specific metabolic depression, and the effect of opioid agonists on metabolism was more pronounced in tissue slices from aestivating animals. Gene transcript levels for the δ-opioid receptor remained constant in the brain and muscle of aestivating C. alboguttata compared with that of active frogs, as did brain transcript levels of the opioid receptor XOR1. The results of this study demonstrate that opioids can influence metabolic rate in isolated tissues from C. alboguttata. The maintenance of key opioid receptor gene transcript levels in tissues of aestivating frogs suggests that despite the energetic costs, maintenance of receptor abundance is important. We propose that the maintenance of opioid receptor abundance during aestivation in C. alboguttata is indicative of a role for the EOS in the regulation of metabolic rate during aestivation.
Keyword Zoology
Zoology
ZOOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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