Comparative analysis of monocyte subsets in the pig

Fairbairn, Lynsey, Kapetanovic, Ronan, Beraldi, Dario, Sester, David P., Tuggle, Chris K., Archibald, Alan L. and Hume, David A. (2013) Comparative analysis of monocyte subsets in the pig. Journal of Immunology, 190 12: 6389-6396. doi:10.4049/jimmunol.1300365

Author Fairbairn, Lynsey
Kapetanovic, Ronan
Beraldi, Dario
Sester, David P.
Tuggle, Chris K.
Archibald, Alan L.
Hume, David A.
Title Comparative analysis of monocyte subsets in the pig
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
Publication date 2013-06-15
Year available 2013
Sub-type Article (original research)
DOI 10.4049/jimmunol.1300365
Volume 190
Issue 12
Start page 6389
End page 6396
Total pages 8
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Subject 2403 Immunology
Abstract Human and mouse monocyte can be divided into two different subpopulations based on surface marker expression: CD14/16 and Ly6C/CX3CR1, respectively. Monocyte subpopulations in the pig were identified based on reciprocal expression of CD14 and the scavenger receptor CD163. The two populations, CD14hi-CD163low and CD14low-CD163hi, show approximately equal abundance in the steady-state. Culture of pig PBMCs in CSF1 indicates that the two populations are a maturation series controlled by this growth factor. Gene expression in pig monocyte subpopulations was profiled using the newly developed and annotated pig whole genome snowball microarray. Previous studies have suggested a functional equivalence between human and mouse subsets, but certain genes such as CD36, CLEC4E, or TREM-1 showed human-specific expression. The same genes were expressed selectively in pig monocyte subsets. However, the profiles suggest that the pig CD14 low-CD163high cells are actually equivalent to intermediate human monocytes, and there is no CD14- CD16+ "nonclassical" population. The results are discussed in terms of the relevance of the pig as a model for understanding human monocyte function. Copyright
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
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