A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium

Barquist, Lars, Langridge, Gemma C., Turner, Daniel J., Phan, Minh-Duy, Turner, A. Keith, Bateman, Alex, Parkhill, Julian, Wain, John and Gardner, Paul P. (2013) A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium. Nucleic Acids Research, 41 8: 4549-4564. doi:10.1093/nar/gkt148


Author Barquist, Lars
Langridge, Gemma C.
Turner, Daniel J.
Phan, Minh-Duy
Turner, A. Keith
Bateman, Alex
Parkhill, Julian
Wain, John
Gardner, Paul P.
Title A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium
Formatted title
A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium
Journal name Nucleic Acids Research   Check publisher's open access policy
ISSN 0305-1048
1362-4954
Publication date 2013-04-01
Year available 2013
Sub-type Article (original research)
DOI 10.1093/nar/gkt148
Open Access Status DOI
Volume 41
Issue 8
Start page 4549
End page 4564
Total pages 16
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
Salmonella Typhi and Typhimurium diverged only ∼50 000 years ago, yet have very different host ranges and pathogenicity. Despite the availability of multiple whole-genome sequences, the genetic differences that have driven these changes in phenotype are only beginning to be understood. In this study, we use transposon-directed insertion-site sequencing to probe differences in gene requirements for competitive growth in rich media between these two closely related serovars. We identify a conserved core of 281 genes that are required for growth in both serovars, 228 of which are essential in Escherichia coli. We are able to identify active prophage elements through the requirement for their repressors. We also find distinct differences in requirements for genes involved in cell surface structure biogenesis and iron utilization. Finally, we demonstrate that transposon-directed insertion-site sequencing is not only applicable to the protein-coding content of the cell but also has sufficient resolution to generate hypotheses regarding the functions of non-coding RNAs (ncRNAs) as well. We are able to assign probable functions to a number of cis-regulatory ncRNA elements, as well as to infer likely differences in trans-acting ncRNA regulatory networks.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
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