Epicuticular compounds of Drosophila subquinaria and D. recens: identification, quantification, and their role in female mate choice

Curtis, Sharon, Sztepanacz, Jacqueline L., White, Brooke E., Dyer, Kelly A., Rundle, Howard D. and Mayer, Paul (2013) Epicuticular compounds of Drosophila subquinaria and D. recens: identification, quantification, and their role in female mate choice. Journal of Chemical Ecology, 39 5: 579-590. doi:10.1007/s10886-013-0284-1


Author Curtis, Sharon
Sztepanacz, Jacqueline L.
White, Brooke E.
Dyer, Kelly A.
Rundle, Howard D.
Mayer, Paul
Title Epicuticular compounds of Drosophila subquinaria and D. recens: identification, quantification, and their role in female mate choice
Formatted title
Epicuticular compounds of Drosophila subquinaria and D. recens: identification, quantification, and their role in female mate choice
Journal name Journal of Chemical Ecology   Check publisher's open access policy
ISSN 0098-0331
1573-1561
Publication date 2013-05-01
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s10886-013-0284-1
Volume 39
Issue 5
Start page 579
End page 590
Total pages 12
Place of publication New York, United States
Publisher Springer
Formatted abstract
The epicuticle of various Drosophila species consists of long-chain cuticular hydrocarbons (CHCs) and their derivatives that play a role in waterproofing and a dynamic means of chemical communication. Here, via gas chromatography and mass spectrometry, we identified and quantified the epicuticular composition of D. recens and D. subquinaria, two closely related species that show a pattern of reproductive character displacement in nature. Twenty-four compounds were identified with the most abundant, 11-cis-Vaccenyl acetate, present only in males of each species. Also exclusive to males were five tri-acylglycerides. The 18 remaining compounds were CHCs, all shared between the sexes and species. These CHCs were composed of odd carbon numbers (C29, C31, C33, and C35), with an increase in structural isomers in the C33 and C35 groups. Saturated hydrocarbons comprise only methyl-branched alkanes and were found only in the C29 and C31 groups. Alkenes were the least prevalent, with alkadienes dominating the chromatographic landscape in the longer chain lengths. Sexual dimorphism was extensive with 6/8 of the logcontrast CHCs differing significantly in relative concentration between males and females in D. recens and D. subquinaria, respectively. Males of the two species also differed significantly in relative concentration of six CHCs, while females differed in none. Female-choice mating trials revealed directional sexual selection on male CHCs in a population of each species, consistent with female mate preferences for these traits. The sexual selection vectors differed significantly in multivariate trait space, suggesting that different pheromone blends determine male attractiveness in each species.
Keyword Cis-vaccenyl acetate
Cuticular hydrocarbons
Gas chromatography
Pheromones
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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