Functional characterization of type-B response regulators in the Arabidopsis cytokinin response

Hill, Kristine, Mathews, Dennis E., Kim, Hyo Jung, Street, Ian H., Wildes, Sarah L., Chiang, Yi-Hsuan, Mason, Michael G., Alonso, Jose M., Ecker, Joseph R., Kieber, Joseph J. and Schaller, G. Eric (2013) Functional characterization of type-B response regulators in the Arabidopsis cytokinin response. Plant Physiology, 162 1: 212-224. doi:10.1104/pp.112.208736


Author Hill, Kristine
Mathews, Dennis E.
Kim, Hyo Jung
Street, Ian H.
Wildes, Sarah L.
Chiang, Yi-Hsuan
Mason, Michael G.
Alonso, Jose M.
Ecker, Joseph R.
Kieber, Joseph J.
Schaller, G. Eric
Title Functional characterization of type-B response regulators in the Arabidopsis cytokinin response
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
1532-2548
Publication date 2013-05-01
Year available 2013
Sub-type Article (original research)
DOI 10.1104/pp.112.208736
Volume 162
Issue 1
Start page 212
End page 224
Total pages 13
Place of publication Rockville, MD, United States
Publisher American Society of Plant Biologists
Language eng
Formatted abstract
Cytokinins play critical roles in plant growth and development, with the transcriptional response to cytokinin being mediated by the type-B response regulators. In Arabidopsis (Arabidopsis thaliana), type-B response regulators (ARABIDOPSIS RESPONSE REGULATORS [ARRs]) form three subfamilies based on phylogenic analysis, with subfamily 1 having seven members and subfamilies 2 and 3 each having two members. Cytokinin responses are predominantly mediated by subfamily 1 members, with cytokinin-mediated effects on root growth and root meristem size correlating with type-B ARR expression levels. To determine which type-B ARRs can functionally substitute for the subfamily 1 members ARR1 or ARR12, we expressed different type-B ARRs from the ARR1 promoter and assayed their ability to rescue arr1 arr12 double mutant phenotypes. ARR1, as well as a subset of other subfamily 1 type-B ARRs, restore the cytokinin sensitivity to arr1 arr12. Expression of ARR10 from the ARR1 promoter results in cytokinin hypersensitivity and enhances shoot regeneration from callus tissue, correlating with enhanced stability of the ARR10 protein compared with the ARR1 protein. Examination of transfer DNA insertion mutants in subfamilies 2 and 3 revealed little effect on several well-characterized cytokinin responses. However, a member of subfamily 2, ARR21, restores cytokinin sensitivity to arr1 arr12 roots when expressed from the ARR1 promoter, indicating functional conservation of this divergent family member. Our results indicate that the type-B ARRs have diverged in function, such that some, but not all, can complement the arr1 arr12 mutant. In addition, our results indicate that type-B ARR expression profiles in the plant, along with posttranscriptional regulation, play significant roles in modulating their contribution to cytokinin signaling.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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