Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis

Koltowska, Katarzyna, Apitz, Holger, Stamataki, Despina, Hirst, Elizabeth M. A., Verkade, Heather, Salecker, Iris and Ober, Elke A. (2013) Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis. Development (Cambridge), 140 9: 1912-1918. doi:10.1242/dev.093583


Author Koltowska, Katarzyna
Apitz, Holger
Stamataki, Despina
Hirst, Elizabeth M. A.
Verkade, Heather
Salecker, Iris
Ober, Elke A.
Title Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis
Journal name Development (Cambridge)   Check publisher's open access policy
ISSN 0950-1991
1477-9129
Publication date 2013-05-01
Year available 2013
Sub-type Article (original research)
DOI 10.1242/dev.093583
Open Access Status DOI
Volume 140
Issue 9
Start page 1912
End page 1918
Total pages 7
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Language eng
Formatted abstract
Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer. Focussing on the liver and eye, we show that zygotic Ssrp1a is essential for proliferation and differentiation during organogenesis. Specifically, gene expression indicative of progressive organ differentiation is disrupted and RNA transcription is globally reduced. Ssrp1a-deficient embryos exhibit DNA synthesis defects and prolonged S phase, uncovering a role distinct from that of Spt16, which promotes G1 phase progression. Gene deletion/replacement experiments in Drosophila show that Ssrp1b, Ssrp1a and N-terminal Ssrp1a, equivalent to the yeast homologue Pob3, can substitute Drosophila Ssrp function. These data suggest that (1) Ssrp1b does not compensate for Ssrp1a loss in the zebrafish embryo, probably owing to insufficient expression levels, and (2) despite fundamental structural differences, the mechanisms mediating DNA accessibility by FACT are conserved between yeast and metazoans. We propose that the essential functions of Ssrp1a in DNA replication and gene transcription, together with its dynamic spatiotemporal expression, ensure organ-specific differentiation and proportional growth, which are crucial for the forming embryo.
Keyword Cell cycle
Drosophila
Hdac
Liver
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID U117581329
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Institute for Molecular Bioscience - Publications
 
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