The function of the conserved regulatory element within the second intron of the mammalian Csf1r locus

Sauter, Kristin A., Bouhlel, M. Amine, O'Neal, Julie, Sester, David P., Tagoh, Hiromi, Ingram, Richard M. I, Pridans, Clare, Bonifer, Constanze and Hume, David A. (2013) The function of the conserved regulatory element within the second intron of the mammalian Csf1r locus. PLoS One, 8 1: . doi:10.1371/journal.pone.0054935


Author Sauter, Kristin A.
Bouhlel, M. Amine
O'Neal, Julie
Sester, David P.
Tagoh, Hiromi
Ingram, Richard M. I
Pridans, Clare
Bonifer, Constanze
Hume, David A.
Title The function of the conserved regulatory element within the second intron of the mammalian Csf1r locus
Formatted title
 The function of the conserved regulatory element within the second intron of the mammalian Csf1r locus
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1371/journal.pone.0054935
Open Access Status DOI
Volume 8
Issue 1
Total pages 12
Place of publication San Francisco, United States
Publisher Public Library of Science
Subject 1100 Agricultural and Biological Sciences
1300 Biochemistry, Genetics and Molecular Biology
2700 Medicine
Abstract The gene encoding the receptor for macrophage colony-stimulating factor (CSF-1R) is expressed exclusively in cells of the myeloid lineages as well as trophoblasts. A conserved element in the second intron, Fms-Intronic Regulatory Element (FIRE), is essential for macrophage-specific transcription of the gene. However, the molecular details of how FIRE activity is regulated and how it impacts the Csf1r promoter have not been characterised. Here we show that agents that down-modulate Csf1r mRNA transcription regulated promoter activity altered the occupancy of key FIRE cis-acting elements including RUNX1, AP1, and Sp1 binding sites. We demonstrate that FIRE acts as an anti-sense promoter in macrophages and reversal of FIRE orientation within its native context greatly reduced enhancer activity in macrophages. Mutation of transcription initiation sites within FIRE also reduced transcription. These results demonstrate that FIRE is an orientation-specific transcribed enhancer element.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number e54935.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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