Striatal cholinergic interneurons display activity-related phosphorylation of ribosomal protein S6

Bertran-Gonzalez, Jesus, Chieng, Billy C., Laurent, Vincent, Valjent, Emmanuel and Balleine, Bernard W. (2012) Striatal cholinergic interneurons display activity-related phosphorylation of ribosomal protein S6. PLoS One, 7 12: . doi:10.1371/journal.pone.0053195

Author Bertran-Gonzalez, Jesus
Chieng, Billy C.
Laurent, Vincent
Valjent, Emmanuel
Balleine, Bernard W.
Title Striatal cholinergic interneurons display activity-related phosphorylation of ribosomal protein S6
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-12-28
Sub-type Article (original research)
DOI 10.1371/journal.pone.0053195
Open Access Status DOI
Volume 7
Issue 12
Total pages 11
Place of publication San Francisco, United States
Publisher Public Library of Science
Subject 1100 Agricultural and Biological Sciences
1300 Biochemistry, Genetics and Molecular Biology
2700 Medicine
Formatted abstract
Cholinergic interneurons (CINs) provide the main source of acetylcholine to all striatal regions, and strongly modulate dopaminergic actions through complex regulation of pre- and post-synaptic acetylcholine receptors. Although striatal CINs have a well-defined electrophysiological profile, their biochemical properties are poorly understood, likely due to their low proportion within the striatum (2-3%). We report a strong and sustained phosphorylation of ribosomal protein S6 on its serine 240 and 244 residues (p-Ser240-244-S6rp), a protein integrant of the ribosomal machinery related to the mammalian target of the rapamycin complex 1 (mTORC1) pathway, which we found to be principally expressed in striatal CINs in basal conditions. We explored the functional relevance of this cellular event by pharmacologically inducing various sustained physiological activity states in CINs and assessing the effect on the levels of S6rp phosphorylation. Cell-attached electrophysiological recordings from CINs in a striatal slice preparation showed an inhibitory effect of tetrodotoxin (TTX) on action potential firing paralleled by a decrease in the p-Ser240-244-S6rp signal as detected by immunofluorescence after prolonged incubation. On the other hand, elevation in extracellular potassium concentration and the addition of apamin generated an increased firing rate and a burst-firing activity in CINs, respectively, and both stimulatory conditions significantly increased Ser240-244-S6rp phosphorylation above basal levels when incubated for one hour. Apamin generated a particularly large increase in phosphorylation that was sensitive to rapamycin. Taken together, our results demonstrate for the first time a link between the state of neuronal activity and a biochemical signaling event in striatal CINs, and suggest that immunofluorescence can be used to estimate the cellular activity of CINs under different pharmacological and/or behavioral conditions. 
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article e53195.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 28 Nov 2013, 20:54:02 EST by System User on behalf of Scholarly Communication and Digitisation Service