The application of biophysical techniques to study antimicrobial peptides

Torcato, Inês M., Castanho, Miguel A. R. B. and Henriques, Sónia T. (2012) The application of biophysical techniques to study antimicrobial peptides. Journal of Spectroscopy, 27 5-6: 541-549. doi:10.1155/2012/460702


Author Torcato, Inês M.
Castanho, Miguel A. R. B.
Henriques, Sónia T.
Title The application of biophysical techniques to study antimicrobial peptides
Journal name Journal of Spectroscopy   Check publisher's open access policy
ISSN 2314-4920
2314-4939
Publication date 2012-01-01
Year available 2012
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1155/2012/460702
Open Access Status DOI
Volume 27
Issue 5-6
Start page 541
End page 549
Total pages 9
Place of publication New York, NY United States
Publisher Hindawi
Language eng
Abstract The increasing bacteria resistance to conventional antibiotics has led to the need for alternative therapies. Being part of the human innate defence system and with a broad spectrum of activity against bacteria, viruses, protozoa, and cancer cells, antimicrobial peptides (AMPs) are a very promising alternative. The mechanism of action of AMPs seems to broadly correlate with their ability to target the bacterial cell membrane. To understand and improve their effect, it is of major importance to unravel their mechanism of action and, in particular, to understand the peptide-membrane binding. Several biophysical techniques such as fluorescence spectroscopy, circular dichroism, zeta potential determination, and atomic force microscopy can be used to achieve this goal. Characteristics of AMPs-membranes interactions and the use of these biophysical techniques will be discussed.
Keyword Biochemical Research Methods
Spectroscopy
Biochemistry & Molecular Biology
Spectroscopy
BIOCHEMICAL RESEARCH METHODS
SPECTROSCOPY
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID PTDC/SAU-BEB/099142/2008
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: School of Biomedical Sciences Publications
 
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