Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach

Violette, Aude, Biass, Daniel, Dutertre, Sebastien, Koua, Dominique, Piquemal, David, Pierrat, Fabien, Stocklin, Reto and Favreau, Philippe (2012) Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach. Journal of Proteomics, 75 17: 5215-5225. doi:10.1016/j.jprot.2012.06.001


Author Violette, Aude
Biass, Daniel
Dutertre, Sebastien
Koua, Dominique
Piquemal, David
Pierrat, Fabien
Stocklin, Reto
Favreau, Philippe
Title Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1874-3919
1876-7737
Publication date 2012-09-18
Sub-type Article (original research)
DOI 10.1016/j.jprot.2012.06.001
Volume 75
Issue 17
Start page 5215
End page 5225
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Predatory marine snails of the genus Conus use venom containing a complex mixture of bioactive peptides to subdue their prey. Here we report on a comprehensive analysis of the protein content of injectable venom from Conus consors, an indo-pacific fish-hunting cone snail. By matching MS/MS data against an extensive set of venom gland transcriptomic mRNA sequences, we identified 105 components out of ~ 400 molecular masses detected in the venom. Among them, we described new conotoxins belonging to the A, M- and O1-superfamilies as well as a novel superfamily of disulphide free conopeptides. A high proportion of the deduced sequences (36%) corresponded to propeptide regions of the A- and M-superfamilies, raising the question of their putative role in injectable venom. Enzymatic digestion of higher molecular mass components allowed the identification of new conkunitzins (~ 7 kDa) and two proteins in the 25 and 50. kDa molecular mass ranges respectively characterised as actinoporin-like and hyaluronidase-like protein. These results provide the most exhaustive and accurate proteomic overview of an injectable cone snail venom to date, and delineate the major protein families present in the delivered venom. This study demonstrates the feasibility of this analytical approach and paves the way for transcriptomics-assisted strategies in drug discovery.
Keyword Conus venom
Peptidomics
Transcriptomics
Conopeptides
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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