Drying and processing protocols affect the quantification of cyanogenic glucosides in forage sorghum

Gleadow, Roslyn M., Moldrup, Morten E., O'Donnell, Natalie H. and Stuart, Peter N. (2012) Drying and processing protocols affect the quantification of cyanogenic glucosides in forage sorghum. Journal of the Science of Food and Agriculture, 92 11: 2234-2238. doi:10.1002/jsfa.5752


Author Gleadow, Roslyn M.
Moldrup, Morten E.
O'Donnell, Natalie H.
Stuart, Peter N.
Title Drying and processing protocols affect the quantification of cyanogenic glucosides in forage sorghum
Journal name Journal of the Science of Food and Agriculture   Check publisher's open access policy
ISSN 0022-5142
1097-0010
Publication date 2012-08-30
Sub-type Article (original research)
DOI 10.1002/jsfa.5752
Open Access Status Not Open Access
Volume 92
Issue 11
Start page 2234
End page 2238
Total pages 5
Place of publication Chichester, West Sussex, United Kingdom
Publisher John Wiley & Sons
Language eng
Formatted abstract
Background: Cyanogenic glucosides are common bioactive products that break down to release toxic hydrogen cyanide (HCN) when combined with specific β-glucosidases. In forage sorghum, high concentrations of the cyanogenic glucoside dhurrin lead to reduced productivity and sometimes death of grazing animals, especially in times of drought, when the dhurrin content of stunted crops is often higher. The aim of this study was to develop harvesting protocols suitable for sampling in remote areas.

Results: Dhurrin concentration in air- and oven-dried leaves was the same as in fresh leaves, with no subsequent losses during storage. Dhurrin concentration was halved when leaves were freeze-dried, although activity of the endogenous dhurrinase was preserved. Direct measurement of dhurrin concentration in methanolic extracts using liquid chromatography/mass spectrometry (LC/MS) gave similar results to methods that captured evolved cyanide. A single freezing event was as effective as fine grinding in facilitating complete conversion of dhurrin to cyanide.

Conclusion: Direct measurement of dhurrin using LC/MS is accurate but expensive and not appropriate for fieldwork. Air drying provides an accurate, low-cost method for preparing tissue for dhurrin analysis, so long as the specific β-glucosidase is added. It is recommended that comparative studies like the one presented here be extended to other cyanogenic species. 
Keyword Cyanogenic glucoside
Dhurrin
Sorghum bicolor
Forage sorghum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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