Loop-mediated isothermal amplification technology: towards point of care diagnostics

Njiru, Zablon Kithinji (2012) Loop-mediated isothermal amplification technology: towards point of care diagnostics. PLoS Neglected Tropical Diseases, 6 6: . doi:10.1371/journal.pntd.0001572

Author Njiru, Zablon Kithinji
Title Loop-mediated isothermal amplification technology: towards point of care diagnostics
Journal name PLoS Neglected Tropical Diseases   Check publisher's open access policy
ISSN 1935-2727
Publication date 2012-06-01
Sub-type Article (original research)
DOI 10.1371/journal.pntd.0001572
Open Access Status DOI
Volume 6
Issue 6
Total pages 4
Place of publication San Francisco, United States
Publisher Public Library of Science
Language eng
Subject 3000 Pharmacology, Toxicology and Pharmaceutics
2739 Public Health, Environmental and Occupational Health
2725 Infectious Diseases
Abstract The invention of the loop-mediated isothermal amplification (LAMP) method a decade ago has given new impetus towards development of point of care diagnostic tests based on amplification of pathogen DNA, a technology that has been the precinct of well-developed laboratories. The LAMP technology amplifies DNA with high sensitivity relying on an enzyme with strand displacement activity under isothermal conditions. Additionally, the technology uses four to six specially designed primers recognising six to eight regions of the target DNA sequence, hence a high specificity. The auto-cycling reactions lead to accumulation of a large amount of the target DNA and other reaction by-products, such as magnesium pyrophosphate, that allow rapid detection using varied formats [1], [2]. Over the last 10 years, LAMP has been used widely in the laboratory setting to detect pathogens of medical and veterinary importance, plant parasitic diseases, genetically modified products, and tumour and embryo sex identification, among other uses [3]. However, its application under field conditions has been limited, partly due to the infancy of the technologies associated with LAMP, such as field-based template preparation methods and product detection formats. In this Viewpoint, the author highlights the essential technologies that require development before the LAMP platform can be progressed into a realistic point of care format for resource-poor endemic areas.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article number e1572

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
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