Gene expression alterations in rocky mountain elk infected with chronic wasting disease

Basu, Urmila, Almeida, Luciane M., Dudas, Sandor, Graham, Catherine E., Czub, Stefanie, Moore, Stephen S. and Guan, Le Luo (2012) Gene expression alterations in rocky mountain elk infected with chronic wasting disease. Prion, 6 3: 282-301. doi:10.4161/pri.19915


Author Basu, Urmila
Almeida, Luciane M.
Dudas, Sandor
Graham, Catherine E.
Czub, Stefanie
Moore, Stephen S.
Guan, Le Luo
Title Gene expression alterations in rocky mountain elk infected with chronic wasting disease
Journal name Prion   Check publisher's open access policy
ISSN 1933-6896
1933-690X United
Publication date 2012-01-01
Year available 2012
Sub-type Article (original research)
DOI 10.4161/pri.19915
Open Access Status DOI
Volume 6
Issue 3
Start page 282
End page 301
Total pages 20
Place of publication Austin, TX United States
Publisher Landes Bioscience
Language eng
Subject 1303 Specialist Studies in Education
1307 Cell Biology
2725 Infectious Diseases
2804 Cellular and Molecular Neuroscience
Abstract Chronic wasting disease (CWD) is an invariably fatal neurologic disease that naturally infects mule deer, white tailed deer and elk. The understanding of CWD neurodegeneration at a molecular level is very limited. In this study, microarray analysis was performed to determine changes in the gene expression profiles in six different tissues including brain, midbrain, thalamus, spleen, RPLN and tonsil of CWD-infected elk in comparison to non-infected healthy elk, using 24,000 bovine specific oligo probes. In total, 329 genes were found to be differentially expressed (> 2.0-fold) between CWD negative and positive brain tissues, with 132 genes upregulated and 197 genes downregulated. There were 249 DE genes in the spleen (168 up and 81 downregulated), 30 DE genes in the retropharyngeal lymph node (RPLN) (18 up and 12 downregulated), and 55 DE genes in the tonsil (21 up and 34 downregulated). Using Gene Ontology (GO), the DE genes were assigned to functional groups associated with cellular process, biological regulation, metabolic process, and regulation of biological process. For all brain tissues, the highest ranking networks for DE genes identified by Ingenuity Pathway Analysis (IPA) were associated with neurological disease, cell morphology, cellular assembly and organization. Quantitative real-time PCR (qRT-PCR) validated the expression of DE genes primarily involved in different regulatory pathways, including neuronal signaling and synapse function, calcium signaling, apoptosis and cell death and immune cell trafficking and inflammatory response. This is the first study to evaluate altered gene expression in multiple organs including brain from orally infected elk and the results will improve our understanding of CWD neurodegeneration at the molecular level.
Keyword Differentially expressed
Microarray
Neurological
Signaling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Alliance for Agriculture and Food Innovation
 
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