DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: Evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide

Santiso, Rebeca, Tamayo, María, Gosalvez, Jaime, Johnston, Steve, Marino, Alfonso, Fernandez, Carlos, Losada, Carlos and Fernandez, Jose Luis (2012) DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: Evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 734 1-2: 41-49. doi:10.1016/j.mrfmmm.2012.03.006


Author Santiso, Rebeca
Tamayo, María
Gosalvez, Jaime
Johnston, Steve
Marino, Alfonso
Fernandez, Carlos
Losada, Carlos
Fernandez, Jose Luis
Title DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: Evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide
Journal name Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
ISSN 0027-5107
1386-1964
1879-2871
Publication date 2012-06-01
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.mrfmmm.2012.03.006
Open Access Status Not Open Access
Volume 734
Issue 1-2
Start page 41
End page 49
Total pages 9
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Subject 1312 Molecular Biology
1311 Genetics
2307 Health, Toxicology and Mutagenesis
Abstract Sperm DNA fragmentation (SDF) is not a static seminal parameter, since the longevity of sperm DNA decreases progressively with time following ejaculation or thawing. While the dynamics of SDF is a species-specific characteristic, in the case of humans, there is still significant variation within patients. To evaluate the suitability of the dynamic SDF assay to assess the adverse effects of agents that cause genetic damage, fresh semen samples from different donors were exposed in vitro to (1) increasing acute doses of ionizing radiation, (2) elevated temperature (41°C and 45°C), (3) acidic pH (pH 4) and (4) the nitric oxide (NO) donor sodium nitroprusside (SNP). Sperm DNA fragmentation was analyzed after an incubation period of chronic (24. h), or acute (1. h) exposure to each treatment followed by incubation at 37°C over a period of 24. h. SDF was assessed using the sperm chromatin dispersion (SCD) test. Dynamic SDF for each treatment was analyzed using Kaplan-Meier survival curves. All agents, except for ionizing radiation, accelerated SDF kinetics following chronic exposure over a 24. h period. Transient exposure to NO and heat but not acidic pH increased the basal (T0) level of SDF. Despite the removal of the three toxicants, the remaining sperm following acute exposure showed a decrease in their expected DNA longevity. It is concluded that the assessment of sperm DNA fragmentation dynamics is an effective methodological approach for revealing latent damage associated with toxicants that is not initially expressed following a single initial observation of SDF.
Keyword DNA fragmentation
Dynamic assay
SCD test, cryptic damage
Sperm
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Agriculture and Food Sciences
 
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