Uncoupled embryonic and extra-embryonic tissues compromise blastocyst development after somatic cell nuclear transfer

Degrelle, Séverine A., Jaffrezic, Florence, Campion, Evelyne, Le Cao, Kim-Anh, Le Bourhis, Daniel, Richard, Christophe, Rodde, Nathalie, Fleurot, Renaud, Everts, Robin E., Lecardonnel, Jerome, Heyman, Yvan, Vignon, Xavier, Yang, Xiangzhong, Tian, Xiuchun C., Lewin, Harris A., Renard, Jean-Paul and Hue, Isabelle (2012) Uncoupled embryonic and extra-embryonic tissues compromise blastocyst development after somatic cell nuclear transfer. PLoS ONE, 7 6: e38309.1-e38309.13. doi:10.1371/journal.pone.0038309

Author Degrelle, Séverine A.
Jaffrezic, Florence
Campion, Evelyne
Le Cao, Kim-Anh
Le Bourhis, Daniel
Richard, Christophe
Rodde, Nathalie
Fleurot, Renaud
Everts, Robin E.
Lecardonnel, Jerome
Heyman, Yvan
Vignon, Xavier
Yang, Xiangzhong
Tian, Xiuchun C.
Lewin, Harris A.
Renard, Jean-Paul
Hue, Isabelle
Title Uncoupled embryonic and extra-embryonic tissues compromise blastocyst development after somatic cell nuclear transfer
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-06-06
Year available 2012
Sub-type Article (original research)
DOI 10.1371/journal.pone.0038309
Open Access Status DOI
Volume 7
Issue 6
Start page e38309.1
End page e38309.13
Total pages 13
Place of publication San Francisco United States
Publisher Public Library of Science (PLoS)
Language eng
Formatted abstract
Somatic cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique available, especially when working with bovine species. Although SCNT blastocysts performed equally well or better than controls in the weeks following embryo transfer at Day 7, elongation and gastrulation defects were observed prior to implantation. To understand the developmental implications of embryonic/extra-embryonic interactions, the morphological and molecular features of elongating and gastrulating tissues were analysed. At Day 18, 30 SCNT conceptuses were compared to 20 controls (AI and IVP: 10 conceptuses each); one-half of the SCNT conceptuses appeared normal while the other half showed signs of atypical elongation and gastrulation. SCNT was also associated with a high incidence of discordance in embryonic and extra-embryonic patterns, as evidenced by morphological and molecular "uncoupling". Elongation appeared to be secondarily affected; only 3 of 30 conceptuses had abnormally elongated shapes and there were very few differences in gene expression when they were compared to the controls. However, some of these differences could be linked to defects in microvilli formation or extracellular matrix composition and could thus impact extra-embryonic functions. In contrast to elongation, gastrulation stages included embryonic defects that likely affected the hypoblast, the epiblast, or the early stages of their differentiation. When taking into account SCNT conceptus somatic origin, i.e. the reprogramming efficiency of each bovine ear fibroblast (Low: 0029, Med: 7711, High: 5538), we found that embryonic abnormalities or severe embryonic/extra-embryonic uncoupling were more tightly correlated to embryo loss at implantation than were elongation defects. Alternatively, extra-embryonic differences between SCNT and control conceptuses at Day 18 were related to molecular plasticity (high efficiency/high plasticity) and subsequent pregnancy loss. Finally, because it alters re-differentiation processes in vivo, SCNT reprogramming highlights temporally and spatially restricted interactions among cells and tissues in a unique way.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
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