Formalin fixation in the '-omics' era: a primer for the surgeon-scientist

Frankel, Adam (2012) Formalin fixation in the '-omics' era: a primer for the surgeon-scientist. ANZ Journal of Surgery, 82 6: 395-402. doi:10.1111/j.1445-2197.2012.06092.x


Author Frankel, Adam
Title Formalin fixation in the '-omics' era: a primer for the surgeon-scientist
Journal name ANZ Journal of Surgery   Check publisher's open access policy
ISSN 1445-1433
1445-2197
Publication date 2012-06-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1111/j.1445-2197.2012.06092.x
Open Access Status Not Open Access
Volume 82
Issue 6
Start page 395
End page 402
Total pages 8
Place of publication Richmond, VIC, Australia
Publisher Wiley-Blackwell
Subject 2746 Surgery
Abstract Formalin is the most commonly used tissue fixative worldwide. While it offers excellent morphological preservation for routine histology, it has detrimental effects on nucleic acids. Most studies of nucleic acids have therefore used fresh frozen tissue, the collection and storage of which is resource intensive. The ability to use modern genomic, transcriptomic and epigenomic methods with nucleic acids derived from formalin-fixed, paraffin-embedded (FFPE) tissues would allow enormous archives of routinely stored tissues (usually with well-annotated clinical data) to be used for translational research. This paper outlines the effects of formalin on nucleic acids, describes ways of minimizing nucleic acid degradation and optimizing extraction, and reviews recent studies that have used contemporary techniques to analyse FFPE-derived nucleic acids (with a focus on malignant tissue sources). Simple tips are also offered to ensure the utility of your institution's samples for future studies, and broadly applicable guidelines are listed for those contemplating their own study using FFPE-derived material.
Keyword Molecular biology
Translational research
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 20 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 28 Nov 2013, 15:15:42 EST by System User on behalf of Scholarly Communication and Digitisation Service