Understanding and avoiding transgene silencing

Graham, Michael W., Mudge, Stephen R., Sternes, Peter R. and Birch, Robert G. (2011). Understanding and avoiding transgene silencing. In C. Neal Stewart, Alisher Touraev, Vitaly Citovsky and Tzvi Tzfira (Ed.), Plant Transformation Technologies (pp. 169-196) Chichester, West Sussex, UK: Blackwell Publishing. doi:10.1002/9780470958988.ch12


Author Graham, Michael W.
Mudge, Stephen R.
Sternes, Peter R.
Birch, Robert G.
Title of chapter Understanding and avoiding transgene silencing
Title of book Plant Transformation Technologies
Place of Publication Chichester, West Sussex, UK
Publisher Blackwell Publishing
Publication Year 2011
Sub-type Research book chapter (original research)
DOI 10.1002/9780470958988.ch12
ISBN 9780813821955
9780470958988
9780470958872
9780470958940
Editor C. Neal Stewart
Alisher Touraev
Vitaly Citovsky
Tzvi Tzfira
Chapter number 12
Start page 169
End page 196
Total pages 28
Total chapters 16
Language eng
Subjects 1100 Agricultural and Biological Sciences
Formatted Abstract/Summary
This chapter describes factors thought to contribute to transgene silencing (such as eukaryotic genome defence systems based on RNA interference, threshold effects and aberrant transcripts, position effects, transgene copy number, transgene integration patterns and read-through, transgene delivery mechanism, and recipient species and development stage) and strategies to avoid the problem based on current understanding of silencing mechanisms. Studies on Arabidopsis thaliana are largely covered.
Keyword Factors influencing transgene silencing
Independent transgenic lines - patterns of transgene expression from same gene construct
Recombinase-mediated resolution - of complex integration events
Threshold effects, and aberrant transcripts - primary triggers
Transcriptional gene silencing (TGS) blocks - and posttranscriptional gene silencing (PTGS)
Transgene expression in plants
Transgene integration in plants - and nonhomologous integration
Transgene integration patterns - and read-through
Transgene integration, stability, methylation and silencing
Understanding and avoiding transgene silencing
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Book Chapter
Collection: School of Biological Sciences Publications
 
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