A conserved GA element in Tata-Less RNA polymerase II promoters

Seizl, Martin, Hartmann, Holger, Hoeg, Friederike, Kurth, Fabian, Martin, Dietmar E., Soding, Johannes and Cramer, Patrick (2011) A conserved GA element in Tata-Less RNA polymerase II promoters. PLoS One, 6 11: . doi:10.1371/journal.pone.0027595


Author Seizl, Martin
Hartmann, Holger
Hoeg, Friederike
Kurth, Fabian
Martin, Dietmar E.
Soding, Johannes
Cramer, Patrick
Title A conserved GA element in Tata-Less RNA polymerase II promoters
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-11-16
Sub-type Article (original research)
DOI 10.1371/journal.pone.0027595
Open Access Status DOI
Volume 6
Issue 11
Total pages 8
Place of publication San Francisco, United States
Publisher Public Library of Science
Subject 1100 Agricultural and Biological Sciences
1300 Biochemistry, Genetics and Molecular Biology
2700 Medicine
Formatted abstract
Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a TATA box occurs in only 15% of promoters in the yeast Saccharomyces cerevisiae, posing the question how most yeast promoters nucleate PIC assembly. Here we show that one third of all yeast promoters contain a novel conserved DNA element, the GA element (GAE), that generally does not co-occur with the TATA box. The distance of the GAE to the transcription start site (TSS) resembles the distance of the TATA box to the TSS. The TATA-less TMT1 core promoter contains a GAE, recruits TBP, and supports formation of a TBP-TFIIB-DNA-complex. Mutation of the promoter region surrounding the GAE abolishes transcription in vivo and in vitro. A 32-nucleotide promoter region containing the GAE can functionally substitute for the TATA box in a TATA-containing promoter. This identifies the GAE as a conserved promoter element in TATA-less promoters. 
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article e27595.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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