Bromo-oxidation reaction in enzyme-entrapped alginate hollow microfibers

Asthana, Amit, Lee, Kwang Ho, Shin, Su-Jung, Perumal, Jayakumar, Butler, Lauren, Lee, Sang-Hoon and Kim, Dong-Pyo (2011) Bromo-oxidation reaction in enzyme-entrapped alginate hollow microfibers. Biomicrofluidics, 5 2: 024117.1-024117.11. doi:10.1063/1.3605512


Author Asthana, Amit
Lee, Kwang Ho
Shin, Su-Jung
Perumal, Jayakumar
Butler, Lauren
Lee, Sang-Hoon
Kim, Dong-Pyo
Title Bromo-oxidation reaction in enzyme-entrapped alginate hollow microfibers
Journal name Biomicrofluidics   Check publisher's open access policy
ISSN 1932-1058
Publication date 2011-04-01
Sub-type Article (original research)
DOI 10.1063/1.3605512
Open Access Status DOI
Volume 5
Issue 2
Start page 024117.1
End page 024117.11
Total pages 11
Place of publication College Park, MD, United States
Publisher American Institute of Physics
Language eng
Formatted abstract
In this article, the authors present the fabrication of an enzyme-entrapped alginate hollow fiber using a microfluidic device. Further use of enzyme-entrapped alginate hollow fibers as a biocatalytic microchemical reactor for chemical synthesis is also deliberated in this article. To ensure that there is no enzyme leaching from the fiber, fiber surfaces were coated with chitosan. To confine the mobility of reactants and products within the porous hollow fibers the entire fibers were embedded into a transparent polydimethylsiloxane (PDMS) matrix which also works as a support matrix. A vanadium-containing bromoperoxidase enzyme isolated from Corallina confusa was used as a model enzyme to demonstrate the use of these alginate hollow-fiber reactors in bromo-oxidation of phenol red to bromophenol blue at different dye flow rates. Stability of the entrapped enzyme at different temperatures and the effect of the chitosan coating on the reaction conversion were also studied. It was observed that molecules as big as 27 kDa can be retained in the matrix after coating with chitosan while molecules with molecular-weight of around 378 Da can still diffuse in and out of the matrix. The kinetic conversion rate in this microfluidic bioreactor was more than 41-fold faster when compared with the standard test-tube procedure.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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