Human corneal endothelial cell growth on a silk fibroin membrane

Madden, Peter W., Lai, Jonathan N. X., George, Karina A., Giovenco, Talia, Harkin, Damien G. and Chirila, Traian V. (2011) Human corneal endothelial cell growth on a silk fibroin membrane. Biomaterials, 32 17: 4076-4084. doi:10.1016/j.biomaterials.2010.12.034


Author Madden, Peter W.
Lai, Jonathan N. X.
George, Karina A.
Giovenco, Talia
Harkin, Damien G.
Chirila, Traian V.
Title Human corneal endothelial cell growth on a silk fibroin membrane
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2011-06-01
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2010.12.034
Volume 32
Issue 17
Start page 4076
End page 4084
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Tissue engineering of the cornea could overcome shortages of donor corneas for transplantation and improve quality. Our aim was to grow an endothelial layer on a substratum suitable for transplant. Silkworm (Bombyx mori) fibroin was prepared as 5 μm thick transparent membranes. The B4G12 cell line was used to assess attachment and growth of human corneal endothelial cells on fibroin and compare this with a reference substratum of tissue-culture plastic. To see if cell attachment and proliferation could be improved, we assessed coatings of collagen IV, FNC Coating Mix® and a chondroitin sulphate–laminin mixture. All the coatings improved the final mean cell count, but consistently higher cell densities were achieved on a tissue-culture plastic rather than fibroin substratum. Collagen-coated substrata were the best of both groups and collagen-coated fibroin was comparable to uncoated tissue-culture plastic. Only fibroin with collagen coating achieved cell confluency. Primary human corneal endothelial cells were then grown using a sphere-forming technique and when seeded onto collagen-coated fibroin they grew to confluency with polygonal morphology. We report the first successful growth of primary human corneal endothelial cells on coated fibroin as a step in evaluating fibroin as a substratum for the transplantation of tissue-constructs for endothelial keratoplasty.
Keyword Silk
Fibroin
Cornea
Endothelium
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Medicine Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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