Extracellular proteomes of M-CSF (CSF-1) and GM-CSF-dependent macrophages

Bailey, Mark J., Lacey, Derek C., De Kok, Bernard V.A., Veith, Paul D., Reynolds, Eric C. and Hamilton, John A. (2011) Extracellular proteomes of M-CSF (CSF-1) and GM-CSF-dependent macrophages. Immunology and Cell Biology, 89 2: 283-293. doi:10.1038/icb.2010.92


Author Bailey, Mark J.
Lacey, Derek C.
De Kok, Bernard V.A.
Veith, Paul D.
Reynolds, Eric C.
Hamilton, John A.
Title Extracellular proteomes of M-CSF (CSF-1) and GM-CSF-dependent macrophages
Journal name Immunology and Cell Biology   Check publisher's open access policy
ISSN 0818-9641
1440-1711
Publication date 2011-01-01
Sub-type Article (original research)
DOI 10.1038/icb.2010.92
Open Access Status Not Open Access
Volume 89
Issue 2
Start page 283
End page 293
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Subject 2403 Immunology
1307 Cell Biology
Abstract Macrophage colony-stimulating factor (M-CSF) (also known as CSF-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) have distinct effects on macrophage lineage populations, which are likely to be contributing to their functional heterogeneity. A comparative proteomic analysis of proteins released into culture media from such populations after M-CSF and GM-CSF exposure was carried out. Adherent macrophage populations, termed bone marrow-derived macrophage (BMM) and GM-BMM, were generated after treatment of murine bone marrow precursors with M-CSF and GM-CSF, respectively. Proteins in 16-h serum-free conditioned media (CM) were identified by two-dimensional gel electrophoresis and mass spectrometry. Respective protein profiles from BMM and GM-BMM CM were distinct and there was the suggestion of a switch from primarily signal peptide-driven secretion to non-classical secretion pathways from BMM to GM-BMM. Extracellular expression of cathepsins (lysosomal proteases) and their inhibitors seems to be a characteristic difference between these macrophage cell types with higher levels usually observed in BMM-CM. Furthermore, we have identified a number of proteins in BMM-CM and GM-BMM-CM that could be involved in various tissue regeneration and inflammatory (immune) processes, respectively. The uncharacterized protein C19orf10, a protein found at high levels in the synovial fluid of arthritis patients, was also differentially regulated; its extracellular levels were upregulated in the presence of GM-CSF.
Keyword granulocyte-macrophage colony-stimulating factor (GM-CSF)
macrophage colony-stimulating factor (M-CSF)
macrophages
proteomics
secretion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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