Pulsed nitrogen supply induces dynamic changes in the amino acid compositionand microcystin production of the harmful cyanobacterium Planktothrix agardhii

Van de Waal, Dedmer B., Ferreruela, Gonzalo, Tonk, Linda, Van Donk, Ellen, Huisman, Jef, Visser, Petra M. and Matthijs, Hans C. P. (2010) Pulsed nitrogen supply induces dynamic changes in the amino acid compositionand microcystin production of the harmful cyanobacterium Planktothrix agardhii. FEMS Microbiology Ecology, 74 2: 430-438. doi:10.1111/j.1574-6941.2010.00958.x


Author Van de Waal, Dedmer B.
Ferreruela, Gonzalo
Tonk, Linda
Van Donk, Ellen
Huisman, Jef
Visser, Petra M.
Matthijs, Hans C. P.
Title Pulsed nitrogen supply induces dynamic changes in the amino acid compositionand microcystin production of the harmful cyanobacterium Planktothrix agardhii
Journal name FEMS Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2010-11-01
Sub-type Article (original research)
DOI 10.1111/j.1574-6941.2010.00958.x
Open Access Status Not Open Access
Volume 74
Issue 2
Start page 430
End page 438
Total pages 9
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Formatted abstract
Planktothrix agardhii is a widespread harmful cyanobacterium of eutrophic waters, and can produce the hepatotoxins [Asp3]microcystin-LR and [Asp3]microcystin-RR. These two microcystin variants differ in their first variable amino acid position, which is occupied by either leucine (L) or arginine (R). Although microcystins are extensively investigated, little is known about the mechanisms that determine the production of different microcystin variants. We hypothesize that enhanced nitrogen availability will increase the intracellular content of the nitrogen-rich amino acid arginine, and thereby promote the production of the variant [Asp3]microcystin-RR. To test this hypothesis, we transferred P. agardhii strain 126/3 from nitrogen-replete to nitrogen-deficient conditions, and after 2 weeks of growth under nitrogen deficiency, we added a nitrogen pulse. We found a rapid increase in the cellular nitrogen to carbon ratio and the amino acids aspartic acid and arginine, indicative of cyanophycin synthesis. This was followed by a more gradual increase of the total amino acid content connected to balanced growth. As expected, the [Asp3] microcystin-RR variant increased strongly after the nitrogen pulse, while the [Asp3]microcystin-LR increased to a much lesser extent. We conclude that sudden nitrogen enrichment affects the amino acid composition of harmful cyanobacteria, which, in turn, affects the production and composition of their microcystins.
Keyword Amino acids
Arginine
Carbon to nitrogen ratio
Harmful cyanobacteria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Marine Studies Publications
 
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