Derivation of three new human embryonic stem cell lines

Bradley, Cara K., Chami, Omar, Peura, Teija T., Bosman, Alexis, Dumevska, Biljana, Schmidt, Uli and Stojanov, Tomas (2010) Derivation of three new human embryonic stem cell lines. In Vitro Cellular and Developmental Biology - Animal, 46 3-4: 294-299. doi:10.1007/s11626-010-9298-y

Author Bradley, Cara K.
Chami, Omar
Peura, Teija T.
Bosman, Alexis
Dumevska, Biljana
Schmidt, Uli
Stojanov, Tomas
Title Derivation of three new human embryonic stem cell lines
Journal name In Vitro Cellular and Developmental Biology - Animal   Check publisher's open access policy
ISSN 1071-2690
Publication date 2010-04-01
Year available 2003
Sub-type Article (original research)
DOI 10.1007/s11626-010-9298-y
Open Access Status Not yet assessed
Volume 46
Issue 3-4
Start page 294
End page 299
Total pages 6
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract Human embryonic stem cells are pluripotent cells capable of extensive self-renewal and differentiation to all cells of the embryo proper. Here, we describe the derivation and characterization of three Sydney IVF human embryonic stem cell lines not already reported elsewhere, designated SIVF001, SIVF002, and SIVF014. The cell lines display typical compact colony morphology of embryonic stem cells, have stable growth rates over more than 40 passages and are cytogenetically normal. Furthermore, the cell lines express pluripotency markers including Nanog, Oct4, SSEA3 and Tra-1-81, and are capable of generating teratoma cells derived from each of the three germ layers in immunodeficient mice. These experiments show that the cell lines constitute pluripotent stem cell lines.
Keyword Derivation
Embryonic stem cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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