Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes

Irving-Rodgers, Helen F., Morris, Stephanie, Collett, Rachael A., Peura, Teija T., Davy, Margaret, Thompson, Jeremy G., Mason, Helen D. and Rodgers, Raymond J. (2009) Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes. Human Reproduction, 24 4: 936-944. doi:10.1093/humrep/den447

Author Irving-Rodgers, Helen F.
Morris, Stephanie
Collett, Rachael A.
Peura, Teija T.
Davy, Margaret
Thompson, Jeremy G.
Mason, Helen D.
Rodgers, Raymond J.
Title Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes
Journal name Human Reproduction   Check publisher's open access policy
ISSN 0268-1161
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1093/humrep/den447
Open Access Status Not Open Access
Volume 24
Issue 4
Start page 936
End page 944
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
BACKGROUND The ovarian follicular basal lamina underlies the epithelial membrana granulosa and maintains the avascular intra-follicular compartment. Additional layers of basal lamina occur in a number of pathologies, including pili annulati and diabetes. We previously found additional layers of follicular basal lamina in a significant percentage of healthy bovine follicles. We wished to determine if this phenomenon existed in humans, and if it was related to oocyte function in the bovine.

METHODS AND RESULTS We examined follicles from human ovaries (n = 18) by electron microscopy and found that many follicles had additional layers of basal lamina. Oocytes (n = 222) from bovine follicles with normal or unusual basal laminas were isolated and their ability to undergo in vitro maturation, fertilization and culture to blastocyst was compared. Healthy bovine follicles with a single layer of basal lamina had oocytes with significantly (P < 0.01) greater developmental competence than healthy follicles with additional layers of follicular basal lamina (65% versus 28%).

CONCLUSIONS These findings provide direct evidence that the phenotype of the follicular basal lamina is related to oocyte competence.
Keyword Basal lamina
In vitro production
Oocyte competence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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