Direct ex vivo evaluation of long-lived protective antiviral memory B cell responses against hepatitis B virus

Ward, Scott M., Phalora, Prabhjeet, Bradshaw, Daniel, Leyendeckers, Heike and Klenerman, Paul (2008) Direct ex vivo evaluation of long-lived protective antiviral memory B cell responses against hepatitis B virus. Journal of Infectious Diseases, 198 6: 813-817. doi:10.1086/591094


Author Ward, Scott M.
Phalora, Prabhjeet
Bradshaw, Daniel
Leyendeckers, Heike
Klenerman, Paul
Title Direct ex vivo evaluation of long-lived protective antiviral memory B cell responses against hepatitis B virus
Journal name Journal of Infectious Diseases   Check publisher's open access policy
ISSN 0022-1899
1537-6613
Publication date 2008-09-15
Sub-type Article (original research)
DOI 10.1086/591094
Volume 198
Issue 6
Start page 813
End page 817
Total pages 5
Place of publication Cary, NC, United States
Publisher Oxford University Press
Language eng
Formatted abstract
Background. The frequency of protective antiviral memory B cells after hepatitis B virus (HBV) vaccination is unknown.

Methods. A novel 2-step immunomagnetic protocol to assess the ex vivo frequency of protective HBV surface antigen (HBsAg)—specific memory B cells was used.

Results. HBsAg-specific memory B cells were detected in vaccinated individuals, although at very low frequency (median, 0.2% of CD19+ cells [range, 0%–4% of CD19+ cells]). No correlation existed between the frequency of HBsAg-specific memory B cells and the corresponding serum antibody titer or B cell enzyme-linked immunosorbent spot findings.

Conclusion. Our results indicate sustained B cell—mediated protection against HBV despite waning antibody titers, which is consistent with clinical observations.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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