Arabidopsis thaliana Y-family DNA polymerase η catalyses translesion synthesis and interacts functionally with PCNA2

Anderson, Heather J., Vonarx, Edward J., Pastushok, Landon, Nakagawa, Mayu, Katafuchi, Atsushi, Gruz, Petr, Di Rubbo, Antonio, Grice, Desma M., Osmond, Megan J., Sakamoto, Ayako N., Nohmi, Takehiko, Xiao, Wei and Kunz, Bernard A. (2008) Arabidopsis thaliana Y-family DNA polymerase η catalyses translesion synthesis and interacts functionally with PCNA2. Plant Journal, 55 6: 895-908. doi:10.1111/j.1365-313X.2008.03562.x


Author Anderson, Heather J.
Vonarx, Edward J.
Pastushok, Landon
Nakagawa, Mayu
Katafuchi, Atsushi
Gruz, Petr
Di Rubbo, Antonio
Grice, Desma M.
Osmond, Megan J.
Sakamoto, Ayako N.
Nohmi, Takehiko
Xiao, Wei
Kunz, Bernard A.
Title Arabidopsis thaliana Y-family DNA polymerase η catalyses translesion synthesis and interacts functionally with PCNA2
Formatted title
Arabidopsis thaliana Y-family DNA polymerase η catalyses translesion synthesis and interacts functionally with PCNA2
Journal name Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2008-09-01
Sub-type Article (original research)
DOI 10.1111/j.1365-313X.2008.03562.x
Volume 55
Issue 6
Start page 895
End page 908
Total pages 14
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Upon blockage of chromosomal replication by DNA lesions, Y-family polymerases interact with monoubiquitylated proliferating cell nuclear antigen (PCNA) to catalyse translesion synthesis (TLS) and restore replication fork progression. Here, we assessed the roles of Arabidopsis thaliana POLH, which encodes a homologue of Y-family polymerase η (Polη), PCNA1 and PCNA2 in TLS-mediated UV resistance. A T-DNA insertion in POLH sensitized the growth of roots and whole plants to UV radiation, indicating that AtPolη contributes to UV resistance. POLH alone did not complement the UV sensitivity conferred by deletion of yeast RAD30, which encodes Polη, although AtPolη exhibited cyclobutane dimer bypass activity in vitro, and interacted with yeast PCNA, as well as with Arabidopsis PCNA1 and PCNA2. Co-expression of POLH and PCNA2, but not PCNA1, restored normal UV resistance and mutation kinetics in the rad30 mutant. A single residue difference at site 201, which lies adjacent to the residue (lysine 164) ubiquitylated in PCNA, appeared responsible for the inability of PCNA1 to function with AtPolη in UV-treated yeast. PCNA-interacting protein boxes and an ubiquitin-binding motif in AtPolη were found to be required for the restoration of UV resistance in the rad30 mutant by POLH and PCNA2. These observations indicate that AtPolη can catalyse TLS past UV-induced DNA damage, and links the biological activity of AtPolη in UV-irradiated cells to PCNA2 and PCNA- and ubiquitin-binding motifs in AtPolη.
Keyword Polymerase η
Proliferating cell nuclear antigen
Translesion synthesis
Ubiquitin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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