Rab14 regulates apical targeting in polarized epithelial cells

Kitt, Khameeka N., Hernandez-Deviez, Delia, Ballantyne, Sarah D., Spiliotis, Elias T., Casanova, James E. and Wilson, Jean M. (2008) Rab14 regulates apical targeting in polarized epithelial cells. Traffic, 9 7: 1218-1231. doi:10.1111/j.1600-0854.2008.00752.x


Author Kitt, Khameeka N.
Hernandez-Deviez, Delia
Ballantyne, Sarah D.
Spiliotis, Elias T.
Casanova, James E.
Wilson, Jean M.
Title Rab14 regulates apical targeting in polarized epithelial cells
Journal name Traffic   Check publisher's open access policy
ISSN 1398-9219
1600-0854
Publication date 2008-07-01
Sub-type Article (original research)
DOI 10.1111/j.1600-0854.2008.00752.x
Volume 9
Issue 7
Start page 1218
End page 1231
Total pages 14
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Epithelial cells display distinct apical and basolateral membrane domains, and maintenance of this asymmetry is essential to the function of epithelial tissues. Polarized delivery of apical and basolateral membrane proteins from the trans Golgi network (TGN) and/or endosomes to the correct domain requires specific cytoplasmic machinery to control the sorting, budding and fission of vesicles. However, the molecular machinery that regulates polarized delivery of apical proteins remains poorly understood. In this study, we show that the small guanosine triphosphatase Rab14 is involved in the apical targeting pathway. Using yeast two-hybrid analysis and glutathione S-transferase pull down, we show that Rab14 interacts with apical membrane proteins and localizes to the TGN and apical endosomes. Overexpression of the GDP mutant form of Rab14 (S25N) induces an enlargement of the TGN and vesicle accumulation around Golgi membranes. Moreover, expression of Rab14-S25N results in mislocalization of the apical raft-associated protein vasoactive intestinal peptide/MAL to the basolateral domain but does not disrupt basolateral targeting or recycling. These data suggest that Rab14 specifically regulates delivery of cargo from the TGN to the apical domain.
Keyword Apical targeting
Epithelial cells
Polarity
Rab14
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
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