Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins

Tongchusak, Songsak, Brusic, Vladimir and Chaiyaroj, Sansanee C. (2008) Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins. Infection, Genetics and Evolution, 8 4: 467-473. doi:10.1016/j.meegid.2007.09.006


Author Tongchusak, Songsak
Brusic, Vladimir
Chaiyaroj, Sansanee C.
Title Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins
Journal name Infection, Genetics and Evolution   Check publisher's open access policy
ISSN 1567-1348
1567-7257
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.meegid.2007.09.006
Volume 8
Issue 4
Start page 467
End page 473
Total pages 7
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Subject 1105 Dentistry
1311 Genetics
2404 Microbiology
2725 Infectious Diseases
Abstract Candida albicans is one of the most important opportunistic dimorphic fungi responsible for hospital acquired fungal infection in humans. Candida infection rarely occurs in healthy individuals but it is frequently associated with patients who suffer from acquired immunodeficiency syndromes. To date, there is no effective vaccine against this fungal infection. Herein we demonstrated the use of immunomics to characterize promiscuous T cell epitope of C. albicans virulence factors by utilizing CandiVF, a C. albicans database previously constructed to be equipped with protein sequence analysis tool, three dimensional structure visualization software, sequence variable analysis program and Hotspot Hunter epitope prediction tool. Secretory aspartyl proteinase (Sap) family was chosen as a model to validate the Hotspot Hunter prediction. Analysis of Saps1-10 protein entries from CandiVF database revealed that a consensus T cell epitope was located at the C-terminal region of Saps1-10. The result of the in silico prediction was subsequently validated by conventional immunological methods. By using overlapping peptides span the predicted consensus T cell epitopes of Saps1-10 as stimulators, it was demonstrated that peptides S6 and S7 could stimulate PBMC proliferation in 9 of 12 blood donors. Interestingly, S2, the predicted T cell epitope of Sap2, was able to induce proliferation of all donors' PBMC. ELISpot assay for the detection of gamma-interferon producing clones confirmed that the peptide S2 actually stimulated T cell proliferation. The results suggest that S2 might be a potential candidate for vaccine development against C. albicans infection or to be utilized as an adjuvant to stimulate the pre-existing CD4+ T cell in other vaccine development.
Keyword Antigenic prediction
Candida albicans
Immunomics
Interferon ELISpot assay
Secretory aspartyl proteinase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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