Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum

Rekangalt D., Verner M.-C., Kues U., Walser P.J., Marmeisse R., Debaud J.-C. and Fraissinet-Tachet L. (2007) Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum. FEMS Microbiology Letters, 268 1: 67-72. doi:10.1111/j.1574-6968.2006.00564.x


Author Rekangalt D.
Verner M.-C.
Kues U.
Walser P.J.
Marmeisse R.
Debaud J.-C.
Fraissinet-Tachet L.
Title Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1111/j.1574-6968.2006.00564.x
Volume 268
Issue 1
Start page 67
End page 72
Total pages 6
Subject 1311 Genetics
1312 Molecular Biology
2402 Applied Microbiology and Biotechnology
2404 Microbiology
Abstract The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.
Keyword Ectomycorrhiza
Green fluorescent protein
Hebeloma cylindrosporum
Reporter gene
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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