Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation

Ngo, Dai-Hung, Ryu, BoMi and Kim, Se-Kwon (2014) Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation. Food Chemistry, 143 246-255. doi:10.1016/j.foodchem.2013.07.067


Author Ngo, Dai-Hung
Ryu, BoMi
Kim, Se-Kwon
Title Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation
Journal name Food Chemistry   Check publisher's open access policy
ISSN 0308-8146
1873-7072
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.foodchem.2013.07.067
Volume 143
Start page 246
End page 255
Total pages 10
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
Skin gelatin of skate (Okamejei kenojei) was hydrolyzed using Alcalase, flavourzyme, Neutrase and protamex. It was found that the Alcalase hydrolysate exhibited the highest angiotensin-I converting enzyme (ACE) inhibitory activity. Then, Alcalase hydrolysate was further hydrolyzed with protease and separated by an ultrafiltration membrane system. Finally, two peptides responsible for ACE inhibitory activity were identified to be MVGSAPGVL (829 Da) and LGPLGHQ (720 Da), with IC50 values of 3.09 and 4.22 μM, respectively. Moreover, the free radical-scavenging activity of the purified peptides was determined in human endothelial cells. In addition, the antioxidative mechanism of the purified peptides was evaluated by protein and gene expression levels of antioxidant enzymes. The current study demonstrated that the peptides derived from skate skin gelatin could be used in the food industry as functional ingredients with potent antihypertensive and antioxidant benefits.
Keyword Bioactive peptides
Antioxidative enzymes
Human endothelial
Skate skin
Spontaneously Hypertensive Rats
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Pharmacy Publications
 
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