Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta

Patel, Jatin, Seppanen, Elke, Chong, Mark S. K., Yeo, Julie S. L., Teo, Erin Y. L., Chan, Jerry K. Y., Fisk, Nicholas M. and Khosrotehrani, Kiarash (2013) Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta. Stem Cells Translational Medicine, 2 11: 839-847. doi:10.5966/sctm.2013-0092

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Patel, Jatin
Seppanen, Elke
Chong, Mark S. K.
Yeo, Julie S. L.
Teo, Erin Y. L.
Chan, Jerry K. Y.
Fisk, Nicholas M.
Khosrotehrani, Kiarash
Title Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta
Journal name Stem Cells Translational Medicine   Check publisher's open access policy
ISSN 2157-6564
2157-6580
Publication date 2013-11-01
Year available 2013
Sub-type Article (original research)
DOI 10.5966/sctm.2013-0092
Open Access Status DOI
Volume 2
Issue 11
Start page 839
End page 847
Total pages 9
Place of publication Durham, NC, United States
Publisher AlphaMed Press
Language eng
Abstract The term placenta is a highly vascularized tissue and is usually discarded upon birth. Our objective was to isolate clinically relevant quantities of fetal endothelial colony-forming cells (ECFCs) from human term placenta and to compare them to the well-established donor-matched umbilical cord blood (UCB)-derived ECFCs. A sorting strategy was devised to enrich for CD45-CD34 CD31Lo cells prior to primary plating to obtain pure placental ECFCs (PL-ECFCs) upon culture. UCB-ECFCs were derived using a well-described assay. PL-ECFCs were fetal in origin and expressed the same cell surface markers as UCB-ECFCs. Most importantly, a single term placenta could yield as many ECFCs as 27 UCB donors. PL-ECFCs and UCB-ECFCs had similar in vitro and in vivo vessel forming capacities and restored mouse hind limb ischemia in similar proportions. Gene expression profiles were only minimally divergent between PL-ECFCs and UCB-ECFCs, probably reflecting a vascular source versus a circulating source. Finally, PL-ECFCs and UCB-ECFCs displayed similar hierarchies between high and low proliferative colonies. We report a robust strategy to isolate ECFCs from human term placentas based on their cell surface expression. This yielded much larger quantities of ECFCs than UCB, but the cells were comparable in immunophenotype, gene expression, and in vivo functional ability. We conclude that PL-ECFCs have significant bio-banking and clinical translatability potential.
Keyword Endothelial cell
Placenta
Progenitor cells
Angiogenesis
Progenitor cells
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID APP1023368
NMRC/1179/2008
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 24 Nov 2013, 10:12:30 EST by System User on behalf of UQ Centre for Clinical Research