Depot- and sex-specific differences in human leptin mRNA expression - Implications for the control of regional fat distribution

Montague, CT, Prins, JB, Sanders, L, Digby, JE and ORahilly, S (1997) Depot- and sex-specific differences in human leptin mRNA expression - Implications for the control of regional fat distribution. Diabetes, 46 3: 342-347. doi:10.2337/diabetes.46.3.342


Author Montague, CT
Prins, JB
Sanders, L
Digby, JE
ORahilly, S
Title Depot- and sex-specific differences in human leptin mRNA expression - Implications for the control of regional fat distribution
Journal name Diabetes   Check publisher's open access policy
ISSN 0012-1797
Publication date 1997-03-01
Year available 1997
Sub-type Article (original research)
DOI 10.2337/diabetes.46.3.342
Open Access Status Not yet assessed
Volume 46
Issue 3
Start page 342
End page 347
Total pages 6
Place of publication ALEXANDRIA
Publisher AMER DIABETES ASSOC
Language eng
Abstract Obese subjects with excess intra-abdominal fat deposition suffer greater adverse metabolic consequences than do similarly overweight subjects with a predominantly subcutaneous distribution of adiposity. Little is known about the factors regulating the regional distribution of body fat. Leptin is a recently characterized protein secreted by adipocytes that appears to provide a long-term hormonal feedback signal regulating fat mass. No systematic evaluation of site-related differences in human adipocyte leptin expression has been reported to date. Levels of leptin mRNA were examined by quantitative reverse transcription-polymerase chain reaction in adipocytes isolated from omental and subcutaneous adipose depots of nonobese and mildly obese individuals undergoing elective surgery. In all individuals studied (n = 24), leptin mRNA levels were higher in subcutaneous than in omental adipocytes (P < 0.0001). In contrast, there were no consistent site-specific differences in the expression of glycerol-3-phosphate dehydrogenase mRNA. The subcutaneous-to-omental ratio of leptin mRNA expression was markedly higher in women (5.5 +/- 1.1-fold) than in men (1.9 +/- 0.2-fold) (P < 0.02). A significant relationship between BMI and leptin mRNA expression was demonstrable in the subcutaneous adipocytes of women (P < 0.006). Thus, leptin mRNA appears to be expressed predominantly by subcutaneous adipocytes, particularly in women. These findings suggest a possible role for leptin in the control of adipose tissue distribution and mass.
Keyword Obese Gene-Expression
Adipose-Tissue
Messenger-Rna
Body-Weight
Ob Protein
Women
Rats
Metabolism
Cloning
Mouse
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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