Tumor necrosis factor induces distinct patterns of caspase activation in WEHI-164 cells associated with apoptosis or necrosis depending on cell cycle stage

Faraco, PR, Ledgerwood, EC, Vandenabeele, P, Prins, JB and Bradley, JR (1999) Tumor necrosis factor induces distinct patterns of caspase activation in WEHI-164 cells associated with apoptosis or necrosis depending on cell cycle stage. Biochemical and Biophysical Research Communications, 261 2: 385-392. doi:10.1006/bbrc.1999.1042


Author Faraco, PR
Ledgerwood, EC
Vandenabeele, P
Prins, JB
Bradley, JR
Title Tumor necrosis factor induces distinct patterns of caspase activation in WEHI-164 cells associated with apoptosis or necrosis depending on cell cycle stage
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 1999-08-01
Year available 1999
Sub-type Article (original research)
DOI 10.1006/bbrc.1999.1042
Open Access Status
Volume 261
Issue 2
Start page 385
End page 392
Total pages 8
Place of publication SAN DIEGO
Publisher ACADEMIC PRESS INC
Language eng
Abstract TNF is unusual among the death receptor ligands in being able to induce either apoptotic or necrotic cell death. We have observed that in WEHI 164 fibrosarcoma, cells the mode of TNF-induced cell death is dependent on the stage of the cell cycle. Cells arrested in G(0)/G(1) undergo necrosis, while those progressing through the cell cycle undergo apoptosis. TNF induces caspase activity in both settings, and the broad spectrum caspase inhibitor zVAD-fmk inhibits this activity and blocks both TNF-induced apoptosis and necrosis. Inhibition of oxygen radical accumulation does not block cytotoxicity, The presence and activation of specific caspases were examined by Western blotting. The procaspase-8a isoform was down-regulated in proliferating cells. Procaspases-8b and -7 were cleaved during TNF-induced apoptosis but not necrosis. Thus, a different pattern of caspase expression and activation occurs dependent on the cell cycle and which may determine the mode of cell death. (C) 1999 Academic Press.
Keyword Fas-Mediated Apoptosis
Death Domain
Tnf
Involvement
Receptors
Protease
Cytotoxicity
Proteolysis
Initiation
Inhibition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 23 Nov 2013, 04:13:07 EST by System User