CD 133(+) and CXCR4(+) colon cancer cells as a marker for lymph node metastasis

Silinsky, Jennifer, Grimes, Chelsea, Driscoll, Tiffany, Green, Heather, Cordova, Jose, Davis, Nancy K., Li, Li and Margolin, David A. (2013) CD 133(+) and CXCR4(+) colon cancer cells as a marker for lymph node metastasis. Journal of Surgical Research, 185 1: 113-118. doi:10.1016/j.jss.2013.05.049


Author Silinsky, Jennifer
Grimes, Chelsea
Driscoll, Tiffany
Green, Heather
Cordova, Jose
Davis, Nancy K.
Li, Li
Margolin, David A.
Title CD 133(+) and CXCR4(+) colon cancer cells as a marker for lymph node metastasis
Formatted title
CD 133+ and CXCR4+ colon cancer cells as a marker for lymph node metastasis
Journal name Journal of Surgical Research   Check publisher's open access policy
ISSN 0022-4804
1095-8673
Publication date 2013-11-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jss.2013.05.049
Open Access Status
Volume 185
Issue 1
Start page 113
End page 118
Total pages 6
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
Introduction
Colorectal cancer (CRC) stem cells or tumor-initiating cells (Co-TIC) are implicated in both cancer recurrence and extranodal metastasis. CD133 and CXCR4 are specific cell surface markers that are indicators of Co-TIC. The presence of lymph node (LN) metastases is one of the strongest negative prognostic factors for CRC patients. We examined the relationship between the Co-TIC markers CD133 and CXCR4 and LN involvement in CRC.

Methods

CRC cells were isolated via enzymatic digestion. CD133+, CXCR4+, and double-positive CRC cells were detected by fluorescence-activated cell sorting analysis. The percentages of CD133+, CXCR4+, and double-positive cells were identified and correlated to the number and percentage of positive LN on staging.

Results
Twenty-seven samples underwent fluorescence-activated cell sorting analysis. The mean percentage of CD133+ cells was 3.94% (range 0.15%–19.06%). The mean percentage of CXCR4+ cells was 6.15% (range 0%–27.11%). The mean percentage of CD133+CXCR4+ cells was 0.45% (range 0%–2.08%). Thirteen patients had LN metastasis: 8 N1 disease and 5 N2 disease. The correlation coefficients between the percentage of Co-TIC marker–positive cells and percentage of positive LN were r = 0.58 (P = 0.0016) for CD133+ cells, r = 0.36 (P = 0.5868) for CXCR4+ cells, and r = 0.56 (P = 0.0022) for double-positive cells.

Discussion
Our results show CD133+ and CD133+CXCR4+ cancer cells correlate with the presence of LN metastasis in CRC. Further studies will examine whether these markers can give consistent prognostic information and may help to develop novel diagnostic and therapeutic options.
Keyword CD133
CXCR4
Co-TIC
CXCL12
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
 
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