Fusarium oxysporum infection assays in Arabidopsis

Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2013). Fusarium oxysporum infection assays in Arabidopsis. In Mark P. Running (Ed.), G protein-coupled receptor signaling in plants: methods and protocols (pp. 67-72) New York, United States: Humana Press. doi:10.1007/978-1-62703-532-3_7


Author Trusov, Yuri
Chakravorty, David
Botella, Jose Ramon
Title of chapter Fusarium oxysporum infection assays in Arabidopsis
Title of book G protein-coupled receptor signaling in plants: methods and protocols
Language of Book Title eng
Place of Publication New York, United States
Publisher Humana Press
Publication Year 2013
Sub-type Article (original research)
DOI 10.1007/978-1-62703-532-3_7
Open Access Status Not yet assessed
Year available 2013
Series Methods in Molecular Biology
ISBN 9781627035323
9781627035316
ISSN 1064-3745
1940-6029
Editor Mark P. Running
Volume number 1043
Chapter number 7
Start page 67
End page 72
Total pages 6
Total chapters 16
Language eng
Abstract/Summary Increased susceptibility to Fusarium oxysporum is one of the most conspicuous characteristics of the Arabidopsis mutants lacking the heterotrimeric G protein beta and gamma 1 subunits. The molecular mechanisms placing these G proteins in the plant innate immunity network are yet to be discovered. However, a method to test susceptibility to and disease progression of an important plant pathogen, such as F. oxysporum, is of central importance to many plant defense studies. The optimized protocol presented here allows the routine processing and analysis of symptom progression in young Arabidopsis soil-grown seedlings and yields highly reproducible results.
Formatted Abstract/Summary
Increased susceptibility to Fusarium oxysporum is one of the most conspicuous characteristics of the Arabidopsis mutants lacking the heterotrimeric G protein β and γ1 subunits. The molecular mechanisms placing these G proteins in the plant innate immunity network are yet to be discovered. However, a method to test susceptibility to and disease progression of an important plant pathogen, such as F. oxysporum, is of central importance to many plant defense studies. The optimized protocol presented here allows the routine processing and analysis of symptom progression in young Arabidopsis soil-grown seedlings and yields highly reproducible results.
Keyword Fusarium oxysporum
Hemi-biotrophic fungi
Arabidopsis
Plant pathogens
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Figures,Color plates

Document type: Book Chapter
Collections: School of Agriculture and Food Sciences
Official 2014 Collection
 
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