Overexpression of PLCE1 in Kazakh esophageal squamous cell carcinoma: implications in cancer metastasis and aggressiveness

Chen, Yun-Zhao, Cui, Xiao-Bin, Hu, Jian-Ming, Zhang, Wen Jie, Li, Shu-Gang, Yang, Lan, Shen, Xi-Hua, Liu, Chun-Xia, Pan, Qing-Fang, Yu, Shi-Ying, Yuan, Xiang-Lin, Yang, Lei, Gu, Wen-Yi, Chen, Jie-Zhong, Wang, Li-Dong and Li, Feng (2013) Overexpression of PLCE1 in Kazakh esophageal squamous cell carcinoma: implications in cancer metastasis and aggressiveness. Apmis, 121 10: 908-918. doi:10.1111/apm.12095


Author Chen, Yun-Zhao
Cui, Xiao-Bin
Hu, Jian-Ming
Zhang, Wen Jie
Li, Shu-Gang
Yang, Lan
Shen, Xi-Hua
Liu, Chun-Xia
Pan, Qing-Fang
Yu, Shi-Ying
Yuan, Xiang-Lin
Yang, Lei
Gu, Wen-Yi
Chen, Jie-Zhong
Wang, Li-Dong
Li, Feng
Title Overexpression of PLCE1 in Kazakh esophageal squamous cell carcinoma: implications in cancer metastasis and aggressiveness
Journal name Apmis   Check publisher's open access policy
ISSN 0903-4641
1600-0463
Publication date 2013-10-01
Year available 2013
Sub-type Article (original research)
DOI 10.1111/apm.12095
Open Access Status Not yet assessed
Volume 121
Issue 10
Start page 908
End page 918
Total pages 11
Place of publication Malden, MA United States
Publisher Wiley-Blackwell Publishing, Inc
Language eng
Subject 2734 Pathology and Forensic Medicine
2723 Immunology and Allergy
2726 Microbiology (medical)
Abstract Three recent large-scale genome-wide association studies (GWAS) in Chinese Han populations have identified an esophageal squamous cell carcinoma (ESCC) susceptibility locus within phospholipase C epsilon 1 (PLCE1) gene, which encodes a phospholipase involved in intracellular signaling. The expressed PLCE1 in ESCC, however, are inconsistent. This study examined PLCE1 expression by immunohistochemistry (IHC) from 110 ethnic Kazakh ESCC patients and 50 from adjacent normal esophageal tissues (NETs). The expressed PLCE1 was localized in cytoplasm, especially in the peripheral layers of cancer cell nests, which was significantly higher in tumors than in NETs (p < 0.001). Increased expression of PLCE1 was correlated with advanced tumor-node-metastasis (TNM) stages (p = 0.015) and lymph node metastasis (p = 0.003) in patients with ESCC. Of the 110 patients, we examined 50 paired ESCC tissues and corresponding NETs by quantitative RT-PCR (polymerase chain reaction) and the mean mRNA level of PLCE1 in ESCC was 1.85-fold higher compared with those in corresponding NETs (p = 0.0012). Meanwhile, 4 of 5 ESCC cell lines also showed elevated expression of PLCE1 mRNA. Furthermore, elevated expression of PLCE1 mRNA in Kazakh ESCC was associated with its immunoreactivity (ρ = 0.297, p = 0.040), lymph node metastasis (p < 0.001), and advanced TNM stages of ESCC (p = 0.013). To our knowledge, this study demonstrates for the first time that PLCE1 overexpression correlates with lymph node metastasis and advanced TNM stages of Kazakh ESCC, implicating a role of PLCE1 in cancer metastasis and aggressiveness in ethnic Kazakh patients with ESCC. Furthermore, the current findings may warrant investigations into whether inhibiting PLCE1 could be a strategy for targeted anticancer therapy particularly for Kazakh ESCC.
Keyword Kazakh esophageal squamous cell carcinoma
Cancer metastasis
Aggressiveness
Genome wide association
Phospholipase-C Epsilon
Skin Tumor Development
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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