Quantification of D-dimer levels in human saliva

Zhang, Xi, Wan, Yunxia, Cooper-White, Justin, Dimeski, Goce, Atherton, John and Punyadeera, Chamindie (2013) Quantification of D-dimer levels in human saliva. Bioanalysis, 5 18: 2249-2256. doi:10.4155/bio.13.190


Author Zhang, Xi
Wan, Yunxia
Cooper-White, Justin
Dimeski, Goce
Atherton, John
Punyadeera, Chamindie
Title Quantification of D-dimer levels in human saliva
Journal name Bioanalysis   Check publisher's open access policy
ISSN 1757-6180
1757-6199
Publication date 2013-09-01
Year available 2013
Sub-type Article (original research)
DOI 10.4155/bio.13.190
Open Access Status Not yet assessed
Volume 5
Issue 18
Start page 2249
End page 2256
Total pages 8
Place of publication London, United Kingdom
Publisher Future Science
Language eng
Formatted abstract
Background: Plasma D-dimer tests are currently used to exclude deep vein thrombosis and pulmonary embolism. Human saliva has numerous advantages over blood as a diagnostic sample. The aims of our study were to develop a reliable immunoassay to detect D-dimer levels in saliva, and to determine the correlation between salivary and blood D-dimer levels. Results/methodology: Saliva and blood samples were collected from 40 healthy volunteers. We developed a AlphaLISA® immunoassay with acceptable analytical performances to quantify D-dimer levels in the samples. The median salivary D-dimer levels were 138.1 ng/ml (morning) and 140.7 ng/ml (afternoon), and the plasma levels were 75.0 ng/ml. Salivary D-dimer levels did not correlate with plasma levels (p = 0.61). Conclusion: For the first time, we have quantified D-dimer levels and found twofold increase in saliva (p < 0.05) than in plasma. Further studies are required to demonstrate the clinical relevance/utility of salivary D-dimer in patients with confirmed deep vein thrombosis and/or pulmonary embolism.
Keyword Deep-Vein thrombosis
C-Reactive protein
Fibrin D-dimer
Pulmonary-embolism
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID G0000934
068545/Z/02
079895
018996
N01-HC-85079
M01-RR00425
DK063491
108-1080315-0302
LSHG-CT-2006-018947
N01-HC-25195
N02-HL-6-4278
01GS0834
BB/F019394/1
CZB/4/505
G0700704/84698
2001 D 032
050-060-810
918-76-619
175.010.2005.011
014-93-015
050-060-810
HEALTH-F4-2007-201413
QLG2-CT-2002-01254
G20234
U01 DK062418
Institutional Status UQ

 
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