Prostaglandin F-2 alpha-induced nest-building behaviour is associated with increased hypothalamic c-fos and c-jun mRNA expression

Walton, SL, Burne, THJ and Gilbert, CL (2002) Prostaglandin F-2 alpha-induced nest-building behaviour is associated with increased hypothalamic c-fos and c-jun mRNA expression. Journal of Neuroendocrinology, 14 9: 711-723. doi:10.1046/j.1365-2826.2002.00831.x


Author Walton, SL
Burne, THJ
Gilbert, CL
Title Prostaglandin F-2 alpha-induced nest-building behaviour is associated with increased hypothalamic c-fos and c-jun mRNA expression
Journal name Journal of Neuroendocrinology   Check publisher's open access policy
ISSN 0953-8194
Publication date 2002-09-01
Year available 2002
Sub-type Article (original research)
DOI 10.1046/j.1365-2826.2002.00831.x
Open Access Status Not yet assessed
Volume 14
Issue 9
Start page 711
End page 723
Total pages 13
Place of publication HOBOKEN
Publisher WILEY
Language eng
Abstract Intramuscular injection of the naturally occurring prostaglandin F-2alpha (PGF(2alpha)) to sexually mature female pigs induces luteolysis and rapidly elicits a behavioural response consistent with pre-partum nest-building. Intramuscular injection of the synthetic prostaglandin F-2alpha (cloprostenol) also induces luteolysis but no nest-building behaviour is observed. The effects of PGF(2alpha), but not cloprostenol, on nest-building behaviour may be mediated via peripheral PGF(2alpha) receptors (FP) or via direct action on central FP receptors. We have previously shown FP receptor mRNA to be localized in porcine paraventricular nucleus (PVN), supraoptic nucleus (SON) and pars dorso-medialis of the suproptic nucleus (SOD), suprachiasmatic nucleus, choroid plexus and anterior and intermediate pituitary lobes. In this experiment, we examined hypothalamic expression of the immediate early genes c-fos and c-jun mRNA after treatment with PGF(2alpha) or cloprostenol. Twenty-one 8-month-old nulliparous female pigs (gilts) were injected intramuscularly with a luteolytic dose of PGF(2alpha) (15 mg), cloprostenol (175 mug) or saline control, their behaviour was recorded and they were killed 60 min later. Coronal hypothalamic sections and control ovarian tissues were incubated with 45-mer oligonucleotide probes complementary to porcine c-fos and c-jun genes using standard in situ hybridization histochemistry techniques. Significantly higher c-fos and c-jun mRNA expression was found in PGF(2alpha)-treated compared to saline or cloprostenol-treated pigs in the PVN, SON and SOD. Significantly higher c-fos and c-jun mRNA expression was found in corpus lutea of PGF(2alpha) and cloprostenol-treated pigs compared to saline controls. Treatment with PGF(2alpha) induced nest-building behaviour whereas treatment with cloprostenol and saline did not. This suggests that PGF(2alpha), or one of its metabolites, and not cloprostenol, crosses the blood-brain barrier and acts directly on hypothalamic receptors to mediate its effect on nest-building behaviour.
Keyword pig
prostaglandin
hypothalamus
nest-building
behaviour
Bovine Luteal Cells
Protein-Kinase-C
Messenger-Ribonucleic-Acid
Ranging Domestic Pigs
Maternal-Behavior
Pseudopregnant Pigs
Parturient Behavior
Endocrine Changes
Macropus-Eugenii
Hormonal-Control
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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