c-fos mRNA expression associated with PGF(2 alpha)-induced nest-building behaviour in female pigs

Burne, THJ, Murfitt, PJE and Gilbert, CL (2002) c-fos mRNA expression associated with PGF(2 alpha)-induced nest-building behaviour in female pigs. Molecular Brain Research, 104 1: 31-37. doi:10.1016/S0169-328X(02)00199-7


Author Burne, THJ
Murfitt, PJE
Gilbert, CL
Title c-fos mRNA expression associated with PGF(2 alpha)-induced nest-building behaviour in female pigs
Journal name Molecular Brain Research   Check publisher's open access policy
ISSN 0169-328X
Publication date 2002-07-01
Year available 2002
Sub-type Article (original research)
DOI 10.1016/S0169-328X(02)00199-7
Open Access Status Not yet assessed
Volume 104
Issue 1
Start page 31
End page 37
Total pages 7
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract Domestic pigs, Sus scrofa, build a maternal nest on the day before parturition. A model for nest building has been established in pigs, in which exogenously administered prostaglandin (PG) F-2alpha. may be used to elicit nesting behaviour in cyclic, pseudopregnant and pregnant pigs. The central mechanisms mediating this response are unknown. The present study determined regional brain activity using semi-quantitative analysis of c-fos mRNA, after induction of nest-building behaviour by PGF(2alpha) in Large White pseudopregnant pigs. Oestradiol valerate injections (5 mg/day) were given on days 11-15 of the oestrous cycle to induce pseudopregnancy. The pigs were housed individually in pens (2.8 x 1.7 m) containing straw. On the test day (day 46 or 47 of pseudopregnancy) animals were injected with 3 ml saline (n=5) or 15 mg of PGF(2alpha) (Lutalyse, Upjohn; n=6) intramuscularly. Pigs treated with PGF(2alpha), but not saline, displayed bouts of rooting, pawing and gathering straw, which we interpret as nest building behaviour. The pigs were killed 65 min after treatment, which was 30 min after peak nest building activity, and the brain, uterus and ovaries removed for processing using in situ hybridisation. Saline-treated pigs had elevated levels of c-fos mRNA, compared to background, in the pituitary, corpus luteum and uterus, and a lower, but elevated, level of expression in cerebellum, cortex, hippocampus and olfactory bulb. PGF(2alpha)-treated pigs had significantly higher levels of c-fos mRNA expression than saline-treated pigs in the parvocellular and magnocellular regions of the hypothalamic paraventricular nucleus, the supraoptic nucleus (including the pars dorso-medial is), the neural lobe of the pituitary gland and the cerebellum. PGF(2alpha)-treated pigs also had significantly higher c-fos induction in corpus luteum. These data show that the pattern of c-fos mRNA expression in specific brain areas is different between pigs that show PGF(2alpha)-induced nest building and saline-injected controls. (C) 2002 Elsevier Science B.V. All rights reserved.
Keyword prostaglandin F-2 alpha
behaviour
HPA-axis
c-fos
nest building
Space Restriction
Maternal-Behavior
Late Pregnancy
Messenger-Rna
Prostaglandin-F2-Alpha
Brain
Rat
Neurons
Involvement
Immunoreactivity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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