Metabolic adaptation and trophic strategies of soil bacteria--C1- metabolism and sulfur chemolithotrophy in Starkeya novella

Kappler, Ulrike and Nouwens, Amanda S. (2013) Metabolic adaptation and trophic strategies of soil bacteria--C1- metabolism and sulfur chemolithotrophy in Starkeya novella. Frontiers in Microbiology, 4 304: 1-12. doi:10.3389/fmicb.2013.00304


Author Kappler, Ulrike
Nouwens, Amanda S.
Title Metabolic adaptation and trophic strategies of soil bacteria--C1- metabolism and sulfur chemolithotrophy in Starkeya novella
Formatted title
Metabolic adaptation and trophic strategies of soil bacteria—C1- metabolism and sulfur chemolithotrophy in Starkeya novella
Journal name Frontiers in Microbiology   Check publisher's open access policy
ISSN 1664-302X
Publication date 2013-10-17
Sub-type Article (original research)
DOI 10.3389/fmicb.2013.00304
Open Access Status DOI
Volume 4
Issue 304
Start page 1
End page 12
Total pages 12
Editor Per Bengtson
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Language eng
Formatted abstract
The highly diverse and metabolically versatile microbial communities found in soil environments are major contributors to the global carbon, nitrogen, and sulfur cycles. We have used a combination of genome –based pathway analysis with proteomics and gene expression studies to investigate metabolic adaptation in a representative of these bacteria, Starkeya novella, which was originally isolated from agricultural soil. This bacterium was the first facultative sulfur chemolithoautotroph that was isolated and it is also able to grow with methanol and on over 39 substrates as a heterotroph. However, using glucose, fructose, methanol, thiosulfate as well as combinations of the carbon compounds with thiosulfate as growth substrates we have demonstrated here that contrary to the previous classification, S. novella is not a facultative sulfur chemolitho- and methylotroph, as the enzyme systems required for these two growth modes are always expressed at high levels. This is typical for key metabolic pathways. In addition enzymes for various pathways of carbon dioxide fixation were always expressed at high levels, even during heterotrophic growth on glucose or fructose, which suggests a role for these pathways beyond the generation of reduced carbon units for cell growth, possibly in redox balancing of metabolism. Our results then indicate that S. novella, a representative of the Xanthobacteraceae family of methylotrophic soil and freshwater dwelling bacteria, employs a mixotrophic growth strategy under all conditions tested here. As a result the contribution of this bacterium to either carbon sequestration or the release of climate active substances could vary very quickly, which has direct implications for the modeling of such processes if mixotrophy proves to be the main growth strategy for large populations of soil bacteria.
Keyword Carbon metabolism
Methylotrophy
Methanol
Chemolithotrophy
Thiosulfate
Soil bacteria
Starkeya novella
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 25 Oct 2013, 21:04:13 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences