Bioanalytical and chemical assessment of the disinfection by-product formation potential: Role of organic matter

Farre, Maria Jose, Day, Sophie, Neale, Peta A., Stalter, Daniel, Tang, Janet Y. M. and Escher, Beate I. (2013) Bioanalytical and chemical assessment of the disinfection by-product formation potential: Role of organic matter. Water Research, 47 14: 5409-5421. doi:10.1016/j.watres.2013.06.017

Author Farre, Maria Jose
Day, Sophie
Neale, Peta A.
Stalter, Daniel
Tang, Janet Y. M.
Escher, Beate I.
Title Bioanalytical and chemical assessment of the disinfection by-product formation potential: Role of organic matter
Journal name Water Research   Check publisher's open access policy
ISSN 0043-1354
Publication date 2013-09-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.watres.2013.06.017
Open Access Status Not Open Access
Volume 47
Issue 14
Start page 5409
End page 5421
Total pages 13
Place of publication London, United Kingdom
Publisher I W A Publishing
Language eng
Formatted abstract
Disinfection by-products (DBP) formed from natural organic matter and disinfectants like chlorine and chloramine may cause adverse health effects. Here, we evaluate how the quantity and quality of natural organic matter and other precursors influence the formation of DBPs during chlorination and chloramination using a comprehensive approach including chemical analysis of regulated and emerging DBPs, total organic halogen quantification, organic matter characterisation and bioanalytical tools. In vitro bioassays allow us to assess the hazard potential of DBPs early in the chain of cellular events, when the DBPs react with their molecular target(s) and activate stress response and defence mechanisms. Given the reactive properties of known DBPs, a suite of bioassays targeting reactive modes of toxic action including genotoxicity and sensitive early warning endpoints such as protein damage and oxidative stress were evaluated in addition to cytotoxicity. Coagulated surface water was collected from three different drinking water treatment plants, along with reverse osmosis permeate from a desalination plant, and DBP formation potential was assessed after chlorination and chloramination. While effects were low or below the limit of detection before disinfection, the observed effects and DBP levels increased after disinfection and were generally higher after chlorination than after chloramination, indicating that chlorination forms higher concentrations of DBPs or more potent DBPs in the studied waters. Bacterial cytotoxicity, assessed using the bioluminescence inhibition assay, and induction of the oxidative stress response were the most sensitive endpoints, followed by genotoxicity. Source waters with higher dissolved organic carbon levels induced increased DBP formation and caused greater effects in the endpoints related to DNA damage repair, glutathione conjugation/protein damage and the Nrf2 oxidative stress response pathway after disinfection. Fractionation studies indicated that all molecular weight fractions of organic carbon contributed to the DBP formation potential, with the humic rich fractions forming the greatest amount of DBPs, while the low molecular weight fractions formed more brominated DBPs due to the high bromide to organic carbon ratio. The presence of higher bromide concentrations also led to a higher fraction of brominated DBPs as well as proportionally higher effects. This study demonstrates how a suite of analytical and bioanalytical tools can be used to effectively characterise the precursors and formation potential of DBPs.
Keyword In vitro bioassay
Oxidative stress
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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