The purine nucleotides guanine, adenine and inosine are a dietary requirement for optimal growth of black tiger prawn, P. monodon

Hoang Do Huu, Tabrett, Simon, Hoffmann, Klaus, Koeppel, Peter and Barnes, Andrew C. (2013) The purine nucleotides guanine, adenine and inosine are a dietary requirement for optimal growth of black tiger prawn, P. monodon. Aquaculture, 408-409 100-105. doi:10.1016/j.aquaculture.2013.05.023


Author Hoang Do Huu
Tabrett, Simon
Hoffmann, Klaus
Koeppel, Peter
Barnes, Andrew C.
Title The purine nucleotides guanine, adenine and inosine are a dietary requirement for optimal growth of black tiger prawn, P. monodon
Formatted title
The purine nucleotides guanine, adenine and inosine are a dietary requirement for optimal growth of black tiger prawn, P. monodon
Journal name Aquaculture   Check publisher's open access policy
ISSN 0044-8486
1873-5622
Publication date 2013-09-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.aquaculture.2013.05.023
Open Access Status DOI
Volume 408-409
Start page 100
End page 105
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 1104 Aquatic Science
Abstract Prawns have a dietary requirement for nucleotides for optimum growth. Commercial diets and many natural feed ingredients, including fishmeal are high in nucleotides, with guanine and its derivatives contributing the highest proportion of total nucleotide. The aim of this study was to test the role of guanine alone and in combination with other purified nucleotides on growth, survival rate and non-specific immunity of prawns using a supplemented semi-purified diet. Semi-purified diets as basal (or control) and basal diets supplemented with 0.4% GMP, 0.5% GMP and with combinations of 0.4% GMP with 0.1% of each of AMP, CMP, IMP or UMP were used to feed the prawns (initial mean weight of 3.83±0.01g) for 53days. Two positive control diets, one comprising the semi-purified diet supplemented with a commercial nucleotide mixture, Optimun at 2.27% (0.5% total nucleotide) and a commercial diet, Lucky Star, were also included. Supplementation levels were chosen based on the optimal total nucleotide content determined in previous trials with the semi-purified diet. Growth rates of prawn (DGC, %/day) were significantly higher in groups of prawn fed diets supplemented with 0.5% GMP, GMP+AMP, GMP+IMP than prawns fed the basal, 0.4% GMP, and GMP+UMP (p≤0.030). THC were highest in groups of prawns fed diets containing GMP+AMP, GMP+IMP, GMP+CMP and the basal diet supplemented with Optimun (32×106-33×106cells/mL) and were significantly higher than THC in groups of prawns fed basal diet and diet supplemented with 0.4% GMP alone (~26.5×106cells/mL) (p≤0.038). On day 52, concentrations of guanine, adenine, total purine and total nucleotide in prawn diets were highly positively correlated with weight of prawns (p≤0.013), whilst THC was significantly correlated with guanine, total purine and total nucleotide content (p≤0.023). Survival rates of prawns were very high (between 92 and 100%) and did not differ significantly among diet treatments (p=0.449). Purine nucleotides, especially guanine, appear to be critical for optimal growth of black tiger prawn, Penaeus monodon.
Keyword Dietary nucleotide
Penaeus monodon
Guanine
Purines
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
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