Applicability of passive sampling to bioanalytical screening of bioaccumulative chemicals in marine wildlife

Jin, Ling, Gaus, Caroline, van Mourik, Louise and Escher, Beate I. (2013) Applicability of passive sampling to bioanalytical screening of bioaccumulative chemicals in marine wildlife. Environmental Science and Technology, 47 14: 7982-7988. doi:10.1021/es401014b


Author Jin, Ling
Gaus, Caroline
van Mourik, Louise
Escher, Beate I.
Title Applicability of passive sampling to bioanalytical screening of bioaccumulative chemicals in marine wildlife
Journal name Environmental Science and Technology   Check publisher's open access policy
ISSN 0013-936X
1520-5851
Publication date 2013-07-01
Year available 2013
Sub-type Article (original research)
DOI 10.1021/es401014b
Volume 47
Issue 14
Start page 7982
End page 7988
Total pages 7
Place of publication Washington, DC United States
Publisher American Chemical Society
Language eng
Formatted abstract
Quantification of bioaccumulative contaminants in biota is time and cost-intensive and the required extensive cleanup steps make it selective toward targeted chemical groups. Therefore tissue extracts prepared for chemical analysis are not amenable to assess the combined effects of unresolved complex mixtures. Passive equilibrium sampling with polydimethylsiloxane (PDMS) has the potential for unbiased sampling of mixtures, and the PDMS extracts can be directly dosed into cell-based bioassays. The passive sampling approach was tested by exposing PDMS to lipid-rich tissue (dugong blubber; 85% lipid) spiked with a known mixture of hydrophobic contaminants (five congeners of tetra- to octachloro-dibenzo-p-dioxins). The equilibrium was attained within 24 h. Lipid-PDMS partition coefficients (Klip-PDMS) ranged from 20 to 38, were independent of hydrophobicity, and within the range of those previously measured for organochlorine compounds. To test if passive sampling can be combined with bioanalysis without the need for chemical cleanup, spiked blubber-PDMS extracts were dosed into the CAFLUX bioassay, which specifically targets dioxin-like chemicals. Small quantities of lipids coextracted by the PDMS were found to affect the kinetics in the regularly applied 24-h bioassay; however, this effect was eliminated by a longer exposure period (72 h). The validated method was applied to 11 unspiked dugong blubber samples with known (native) dioxin concentrations. These results provide the first proof of concept for linking passive sampling of lipid-rich tissue with cell-based bioassays, and could be further extended to other lipid rich species and a wider range of bioanalytical end points
Keyword Dioxin Like Compounds
Organic Chemicals
Endocrine Disruption
Risk assessment and regulations
Cell Bioassay
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
National Research Centre for Environmental Toxicology Publications
 
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