MicroRNAome of Spodoptera frugiperda cells (Sf9) and its alteration following baculovirus infection

Mehrabadi, Mohammad, Hussain, Mazhar and Asgari, Sassan (2013) MicroRNAome of Spodoptera frugiperda cells (Sf9) and its alteration following baculovirus infection. Journal of General Virology, 94 Part 6: 1385-1397. doi:10.1099/vir.0.051060-0


Author Mehrabadi, Mohammad
Hussain, Mazhar
Asgari, Sassan
Title MicroRNAome of Spodoptera frugiperda cells (Sf9) and its alteration following baculovirus infection
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2013-06-01
Sub-type Article (original research)
DOI 10.1099/vir.0.051060-0
Volume 94
Issue Part 6
Start page 1385
End page 1397
Total pages 13
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Formatted abstract
MicroRNAs (miRNAs) as small non-coding RNAs play important roles in many biological processes such as development, cell signalling and immune response. Studies also suggest that miRNAs are important in host-virus interactions where the host limits virus infection by differentially expressing miRNAs that target essential viral genes. Here, we identified conserved and new miRNAs from Spodoptera frugiperda cells (Sf9) using a combination of deep sequencing and bioinformatics as well as experimental approaches. S. frugiperda miRNAs share common features of miRNAs in other organisms, such as uracil (U) at the 59 end of miRNA. The 59 ends of the miRNAs were more conserved than the 39 ends, revealing evolutionary protection of the seed region in miRNAs. The predominant miRNAs were found to be conserved among arthropods. The majority of homologous miRNAs were found in Bombyx mori, with 76 of the 90 identified miRNAs. We found that seed shifting and arm switching have happened in this insect's miRNAs. Expression levels of the majority of miRNAs changed following baculovirus infection. Results revealed that baculovirus infection mainly led to an overall suppression of cellular miRNAs. We found four different genes being regulated by sfr-miR-184 at the post-transcriptional level. The data presented here further support conservation of miRNAs in insects and other organisms. In addition, the results reveal a differential expression of host miRNAs upon baculovirus infection, suggesting their potential roles in host-virus interactions. Seed shifting and arm switching happened during evolution of miRNAs in different insects and caused miRNA diversification, which led to changes in the target repository of miRNAs.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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