Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons

Anggono, Victor, Cousin, Michael A. and Robinson, Phillip J. (2008). Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons. In Ales Vancura (Ed.), Membrane Trafficking (pp. 333-345) Totowa, NJ. United States: Humana Press. doi:10.1007/978-1-59745-261-8_25


Author Anggono, Victor
Cousin, Michael A.
Robinson, Phillip J.
Title of chapter Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons
Title of book Membrane Trafficking
Place of Publication Totowa, NJ. United States
Publisher Humana Press
Publication Year 2008
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-59745-261-8_25
Open Access Status Not Open Access
Year available 2008
Series Methods in molecular biology, v. 457
ISBN 9781588299253
9781597452618
ISSN 1064-3745
Editor Ales Vancura
Volume number 457
Chapter number 25
Start page 333
End page 345
Total pages 13
Total chapters 28
Language eng
Abstract/Summary Neurons transmit information by exocytosis of synaptic vesicles (SV), which contain neurotransmitter. Exocytosis is followed by efficient retrieval of the plasma membrane by endocytosis to generate a new SV. SV retrieval supports multiple cycles of synaptic transmission. Over the years, styryl dyes have been widely used to probe the mechanism of SV recycling in the processes of cultured neurons. The styryl dye method is complementary to electrophysiological measurements or genetic reporter approaches. Owing to their ease to culture, cerebellar granule neurons provide a robust neuronal model system for the assay. These cells are readily transfected with various DNA constructs to study the function of exocytic or endocytic proteins in SV recycling.
Keyword Cerebellar granule neuron
Endocytosis
Exocytosis
Synaptic vesicle recycling
Styryl dye
Transfection
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
Collection: Queensland Brain Institute Publications
 
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Created: Sun, 08 Sep 2013, 23:49:21 EST by Victor Anggono on behalf of Clem Jones Centre for Ageing Dementia Research