RNA synthesis inhibition stabilises urokinase mRNA in macrophages

Stacey, Katryn J., Nagamine, Yoshikuni and Hume, David A. (1994) RNA synthesis inhibition stabilises urokinase mRNA in macrophages. FEBS Letters, 356 2-3: 311-313. doi:10.1016/0014-5793(94)01294-6

Author Stacey, Katryn J.
Nagamine, Yoshikuni
Hume, David A.
Title RNA synthesis inhibition stabilises urokinase mRNA in macrophages
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 1994-12-19
Sub-type Article (original research)
DOI 10.1016/0014-5793(94)01294-6
Open Access Status Not yet assessed
Volume 356
Issue 2-3
Start page 311
End page 313
Total pages 3
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Language eng
Subject 1303 Biochemistry
1304 Biophysics
1312 Molecular Biology
Abstract Urokinase-type plasminogen activator (uPA) mRNA is induced in macrophages by the lineage specific growth factor CSF-1. Upon removal of CSF-1 from bone marrow-derived macrophages (BMM), uPA mRNA decayed with a half-life of 2 h. If RNA synthesis inhibitors actinomycin D, 5,6-dichloro-1-β-ribofuranosyl benzimidazole (DRB) or α-amanitin were added at the time as CSF-1 removal, the uPA message was stabilised. This was not a general effect on CSF-1 responsive mRNAs, as c-myc mRNA decayed with normal kinetics in the presence of inhibitors. The requirement for ongoing RNA synthesis for the degradation of uPA mRNA in BMM suggests that a component of the degradative pathway may be induced following removal of CSF-1.
Keyword Actinomycin D
mRNA stability
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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Created: Sat, 07 Sep 2013, 03:27:23 EST by Dr Katryn Stacey on behalf of School of Chemistry & Molecular Biosciences