Effects of surface charge and hydrophobicity on anodic biofilm formation, community composition, and current generation in bioelectrochemical systems

Guo, Kun, Freguia, Stefano, Dennis, Paul G., Chen, Xin, Donose, Bogdan C., Keller, Jurg, Gooding, J. Justin and Rabaey, Korneel (2013) Effects of surface charge and hydrophobicity on anodic biofilm formation, community composition, and current generation in bioelectrochemical systems. Environmental Science and Technology, 47 13: 7563-7570. doi:10.1021/es400901u


Author Guo, Kun
Freguia, Stefano
Dennis, Paul G.
Chen, Xin
Donose, Bogdan C.
Keller, Jurg
Gooding, J. Justin
Rabaey, Korneel
Title Effects of surface charge and hydrophobicity on anodic biofilm formation, community composition, and current generation in bioelectrochemical systems
Journal name Environmental Science and Technology   Check publisher's open access policy
ISSN 0013-936X
1520-5851
Publication date 2013-07-01
Year available 2013
Sub-type Article (original research)
DOI 10.1021/es400901u
Open Access Status Not yet assessed
Volume 47
Issue 13
Start page 7563
End page 7570
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract The focus of this study was to investigate the effects of surface charge and surface hydrophobicity on anodic biofilm formation, biofilm community composition, and current generation in bioelectro-chemical systems (BESs). Glassy carbon surfaces were modified with -OH, -CH3, -SO3-, or -N+(CH3)(3) functional groups by electrochemical reduction of aryl diazonium salts and then used as anodes with poised potential of -0.2 V (vs Ag/AgCl). The average startup times and final current densities for the -N+(CH3)(3), -OH, -SO3-, and -CH3, electrodes were (23 d, 0204. rnA/cm(2)), (254 d, 0.149 mA/cm(2)), (25.9 d, 0.114 mA/cm(2)), and (37.2 d, 0.048 mA/cm(2)), respectively. Biofilms on different surfaces were analyzed by nonturnover cyclic voltammetry (CV), fluorescence in situ hybridization (FISH), and 16S rRNA gene amplicon pyrosequencing. The results demonstrated that 1) differences in the maximum current output between surface modifications was correlated with biomass quantity, and 2) all biofilms were dominated by Geobacter populations, but the composition of -CH3-associated biofilms differed from those formed on surfaces with different chemical modification. This study shows that anode surface charge and hydrophobicity influences biofilm development and can lead to significant differences in BESs performance. Positively charged and hydrophilic surfaces were more selective to electroactive microbes (e.g. Geobacter) and more conducive for electroactive biofilm formation.
Formatted abstract
The focus of this study was to investigate the effects of surface charge and surface hydrophobicity on anodic biofilm formation, biofilm community composition, and current generation in bioelectrochemical systems (BESs). Glassy carbon surfaces were modified with -OH, -CH3, -SO3 -, or -N+(CH3)3 functional groups by electrochemical reduction of aryl diazonium salts and then used as anodes with poised potential of -0.2 V (vs Ag/AgCl). The average startup times and final current densities for the -N+(CH3)3, -OH, -SO3 -, and -CH3, electrodes were (23 d, 0.204 mA/cm2), (25.4 d, 0.149 mA/cm2), (25.9 d, 0.114 mA/cm 2), and (37.2 d, 0.048 mA/cm2), respectively. Biofilms on different surfaces were analyzed by nonturnover cyclic voltammetry (CV), fluorescence in situ hybridization (FISH), and 16S rRNA gene amplicon pyrosequencing. The results demonstrated that 1) differences in the maximum current output between surface modifications was correlated with biomass quantity, and 2) all biofilms were dominated by Geobacter populations, but the composition of -CH3-associated biofilms differed from those formed on surfaces with different chemical modification. This study shows that anode surface charge and hydrophobicity influences biofilm development and can lead to significant differences in BESs performance. Positively charged and hydrophilic surfaces were more selective to electroactive microbes (e.g. Geobacter) and more conducive for electroactive biofilm formation.
Keyword Engineering, Environmental
Environmental Sciences
Engineering
Environmental Sciences & Ecology
ENGINEERING, ENVIRONMENTAL
ENVIRONMENTAL SCIENCES
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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