Purification and characterization of wheat and pine small basic-protein substrates for plant calcium-dependent protein kinase

Polya, Gideon M., Chandra, Shubhra, Chung, Roland, Neumann, Gregory M. and Høj, Peter B. (1992) Purification and characterization of wheat and pine small basic-protein substrates for plant calcium-dependent protein kinase. Biochimica Et Biophysica Acta, 1120 3: 273-280. doi:10.1016/0167-4838(92)90248-C


Author Polya, Gideon M.
Chandra, Shubhra
Chung, Roland
Neumann, Gregory M.
Høj, Peter B.
Title Purification and characterization of wheat and pine small basic-protein substrates for plant calcium-dependent protein kinase
Journal name Biochimica Et Biophysica Acta   Check publisher's open access policy
ISSN 0006-3002
Publication date 1992-04-17
Sub-type Article (original research)
DOI 10.1016/0167-4838(92)90248-C
Open Access Status Not Open Access
Volume 1120
Issue 3
Start page 273
End page 280
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract A wheat basic protein (WBP) was purified to homogeneity from wheat germ by a protocol involving extraction, centrifugation, batchwise elution from carboxymethylcellulose (CM-52), acidification with trifluoroacetic acid, neutralization and HPLC on a SP5PW cation exchange column. WBP is a 10 kDa protein and is phosphorylated on serine residues by wheat germ Ca2+-dependent protein kinase (CDPK). [32P]phosphoWBP exactly comigrates with WBP on SDS-PAGE. WBP does not inhibit either wheat germ CDPK or calmodulin-dependent myosin light chain kinase. Apart from histone H1, WBP is the best endogenous substrate yet found for wheat embryo CDPK. A 12 kDa pine basic protein (PBP) was purified to homogeneity from seeds of stone pine (Pinus pinea L.) by a simple procedure involving batchwise elution from carboxymethylcellulose and cation exchange HPLC. PBP is also a good substrate for CDPK and is phosphorylated on Ser residues. N-terminal sequencing of WBP and PBP revealed that these proteins are homologous to a family of small basic plant proteins having a phospholipid transfer function.
Keyword Calcium-dependent protein kinase
Phospholipid transfer protein
Protein purification
Wheat
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Office of the Vice-Chancellor
 
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