A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats

Mugford, Sam T., Qi, Xiaoquan, Bakht, Saleha, Hill, Lionel, Wegel, Eva, Hughes, Richard K., Papadopoulou, Kalliopi, Melton, Rachel, Philo, Mark, Sainsbury, Frank, Lomonossoff, George P., Roy, Abhijeet Deb, Goss, Rebecca J. M. and Osbourn, Anne (2009) A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats. Plant Cell, 21 8: 2473-2484. doi:10.1105/tpc.109.065870


Author Mugford, Sam T.
Qi, Xiaoquan
Bakht, Saleha
Hill, Lionel
Wegel, Eva
Hughes, Richard K.
Papadopoulou, Kalliopi
Melton, Rachel
Philo, Mark
Sainsbury, Frank
Lomonossoff, George P.
Roy, Abhijeet Deb
Goss, Rebecca J. M.
Osbourn, Anne
Title A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats
Journal name Plant Cell   Check publisher's open access policy
ISSN 1040-4651
1532-298X
Publication date 2009-08-01
Year available 2009
Sub-type Article (original research)
DOI 10.1105/tpc.109.065870
Volume 21
Issue 8
Start page 2473
End page 2484
Total pages 12
Place of publication Rockville, MD United States
Publisher American Society of Plant Biologists
Collection year 2009
Language eng
Formatted abstract
Serine carboxypeptidase-like (SCPL) proteins have recently emerged as a new group of plant acyltransferases. These enzymes share homology with peptidases but lack protease activity and instead are able to acylate natural products. Several SCPL acyltransferases have been characterized to date from dicots, including an enzyme required for the synthesis of glucose polyesters that may contribute to insect resistance in wild tomato (Solanum pennellii) and enzymes required for the synthesis of sinapate esters associated with UV protection in Arabidopsis thaliana. In our earlier genetic analysis, we identified the Saponin-deficient 7 (Sad7) locus as being required for the synthesis of antimicrobial triterpene glycosides (avenacins) and for broad-spectrum disease resistance in diploid oat (Avena strigosa). Here, we report on the cloning of Sad7 and show that this gene encodes a functional SCPL acyltransferase, SCPL1, that is able to catalyze the synthesis of both N-methyl anthraniloyl- and benzoyl-derivatized forms of avenacin. Sad7 forms part of an operon-like gene cluster for avenacin synthesis. Oat SCPL1 (SAD7) is the founder member of a subfamily of monocot-specific SCPL proteins that includes predicted proteins from rice (Oryza sativa) and other grasses with potential roles in secondary metabolism and plant defense. 
Keyword Antisweet Natural Products
Plant Secondary Metabolism
Koidz Var Japonica
Brassica napus
Heterologous Expression
Arabidopsis thaliana
Triterpene Saponins
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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