Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants

Saunders, Keith, Sainsbury, Frank and Lomonossoff, George P. (2009) Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants. Virology, 393 2: 329-337. doi:10.1016/j.virol.2009.08.023


Author Saunders, Keith
Sainsbury, Frank
Lomonossoff, George P.
Title Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1096-0341
1089-862X
Publication date 2009-10-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.virol.2009.08.023
Open Access Status DOI
Volume 393
Issue 2
Start page 329
End page 337
Total pages 9
Place of publication Waltham, MA United States
Publisher Academic Press
Language eng
Formatted abstract
To elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and the 24K proteinase itself, while processing of VP60, consisting of the fused L–S protein, required only the 24K proteinase. Release of the L and S proteins resulted in the formation of virus-like particles (VLPs), showing that VP60 can act as a precursor of virus capsids. Processing of VP60 expressed in plants also led to efficient production of VLPs. Analysis of the VLPs produced by the action of the 24K proteinase on precursors showed that they were empty (RNA-free). This has important implications for the use of CPMV VLPs in biotechnology and nanotechnology as it will permit the use of noninfectious particles.
Keyword Cowpea mosaic virus
Virus like particles
Empty particle
Coat protein precursor
Proteolytic processing
Insect cell expression
Transient expression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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